| 杨书红,朱德强,占大钱,魏 双,吕 兴.PARP-1抑制剂3-AB对肝癌细胞增殖及凋亡的影响*[J].,2019,19(1):17-20 |
| PARP-1抑制剂3-AB对肝癌细胞增殖及凋亡的影响* |
| The Effect of PARP-1 Inhibitor 3-AB on Hepatocellular CarcinomaCell Lines* |
| 投稿时间：2018-05-20 修订日期：2018-06-08 |
| DOI：10.13241/j.cnki.pmb.2019.01.004 |
| 中文关键词: PARP-1 3-AB 肝癌细胞 增殖 凋亡 |
| 英文关键词: PARP-1 3-AB Hepatocellular Carcinoma Cell Line Proliferation Apoptosis |
| 基金项目:国家自然科学基金青年基金项目（81501227） |
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| 中文摘要: |
| 摘要 目的：探讨PARP-1抑制剂3-AB对肝癌细胞系MHCC97-H和SMMC7721及正常肝细胞系L02的增殖与凋亡的影响。方法：细胞增殖试验观察不同浓度3-AB对三种不同细胞系细胞的增殖作用。Annexin V荧光探针标记，流式细胞学检查观察不同浓度3-AB对不同细胞系细胞凋亡的影响。结果：当3-AB浓度分别为5 mM、10 mM与20 mM时，与对照组（0 mM）相比，在培养第6天时开始出现增殖明显减慢，出现统计学差异（p<0.05），第九天差异明显（p<0.05）。随着浓度增加，其对肿瘤细胞系MHCC97-H和SMMC7721细胞增殖的抑制程度增加，细胞数均逐渐减少；而同样浓度梯度3-AB对人类肝细胞系L02生长则无明显的抑制作用。进一步实验发现，当3-AB浓度为5mM、10 mM与20 mM时，均可诱导肝癌细胞株MHCC97-H和SMMC7721凋亡，与对照组（0 mM）比较均有统计学差异（p<0.05），且细胞凋亡率与3-AB的药物浓度相关：浓度越高，凋亡越明显。而同等浓度3-AB对肝脏细胞系L02无明显的促进凋亡作用。结论：3-AB可以抑制肝癌肿瘤细胞的增殖，促进肿瘤细胞的凋亡，对正常肝脏细胞无明显毒害作用，具有治疗肝癌的的潜在应用价值。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of parp-1 inhibitor 3-AB on the proliferation and apoptosis of the hepatocellular carcinoma cell lines of MHCC97-H, SMMC7721 and hepatocellular cell line L02. Methods: Cell proliferation assay was conducted to observe the effects of 3-AB on L02, MHCC97-H and SMMC7721 cell growth. FACS was used to evaluate the apoptosis ratio of different cell lines of human liver cancer cell lines and hepatocellular cell line L02 with different 3-AB dosage. Results: 3-AB can significantly inhibit the growth rate of MHCC97-H and SMMC7721 cell lines. As the concentration increased, the suppressive effects of 3-AB were increasing. The growth inhibition was significant on the 6th and 9th day of culture. Compared with the control group(0 mM), the growth rate of MHCC97-H and SMMC7721 cell lines with different concentrations of 3-AB (5 mM, 10 mM and 20 mM)(p<0.05) were lower on the 6th day and from then on; 3-AB did not affect the growth rate of L02 cell line in the same concentration(p>0.05). Compared with the control group(0 mM), the apoptosis ratio of MHCC97-H and SMMC7721 cell lines were higher than the other 3 groups with different concentration of 3-AB (5 mM, 10 mM and 20 mM) (p<0.05). Yet it has no effect on the apoptosis of L02 cell line in the same concentration(p>0.05). Conclusion: 3-AB can inhibit the growth rate of MHCC97-H and SMMC7721 cell lines and induce the cells apoptosis. And the apoptosis ratio was variable with the different 3-AB concentrations. But different concentrations had no effect on the growth or apoptosis of L02 cell line. 3-AB can be a potential good choice for liver cancer therapy and maybe improve the patients life quality. |
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