周金爱,余 军,黄东萍,吴 睿,刘 欣,臧 宁.速生桉叶水溶提取物对HepG2细胞增殖及凋亡的影响[J].,2018,(24):4623-4627 |
速生桉叶水溶提取物对HepG2细胞增殖及凋亡的影响 |
Effects of Leaf Water Extract from Fast-Growing Eucylyptus Robusta on Proliferaton and Apoptosis of Human Heptoma HepG2 Cells |
投稿时间:2018-06-21 修订日期:2018-07-26 |
DOI:10.13241/j.cnki.pmb.2018.24.004 |
中文关键词: 速生桉 HepG2 细胞增殖 细胞凋亡 |
英文关键词: Fast-growing eucylyptus HepG2 Cells Proliferation Apoptosis |
基金项目:广西科学研究与技术开发计划项目(桂科能1598025-12);广西研究生教育创新计划项目(YCSW2018118) |
|
摘要点击次数: 452 |
全文下载次数: 214 |
中文摘要: |
摘要 目的:探讨速生桉叶水溶提取物对HepG2细胞的抑制增殖作用及凋亡的影响。方法:采用MTT法检测速生桉叶水溶提取物对HepG2细胞增殖抑制作用;采用流式细胞仪Annexin V-FITC/PI检测速生桉叶水溶提取物处理后HepG2细胞凋亡的情况。结果:MTT分析显示,当细胞培养24 h时,稀释5、10倍HepG2细胞抑制率分别为(47.32±1.11)%、(15.76±3.50)%;当细胞培养48 h时,稀释5、10倍HepG2细胞抑制率分别为(44.13±10.93)%、(25.93±8.37)%;当细胞培养72 h时,稀释5、10倍HepG2细胞抑制率分别为(59.47±6.90)%、(41.02±4.27)%。流式细胞术结果显示,速生桉叶水溶提取物稀释30倍时可诱导31.03%的HepG2细胞进入早期凋亡阶段,随着稀释倍数的减少被诱导进入凋亡阶段的细胞数目逐渐升高。另外,随着水溶提取物作用时间的延长,进入凋亡阶段的细胞数目也逐渐升高。结论:速生桉叶水溶提取物对HepG2细胞增殖与凋亡具有量-效、时-效关系。 |
英文摘要: |
ABSTRACT Objective: To investigate the effects of the leaf of Fast-growing eucylyptus extract on the proliferation and apoptosis of human hepatoma HepG2 cells. Methods: MTT assay was used to measure the leaf of Fast-growing eucylyptus extract on HepG2 cells activity. The cell apoptosis of HepG2 cells were determined by flow cytometry Annexin V-FITC/PI. Results: As the results of MTT showed, HepG2 cells were cultured for 24 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were (47.32±1.11) % and (15.76 ±3.50) %, respectively. When HepG2 cells were cultured for 48 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were respectively (44.13±10.93) % and (25.93±8.37) %. When HepG2 cells were cultured for 72 h, the inhibition rate of HepG2 cells diluted 5 and 10 times was (59.47±6.90) % and (41.02±4.27) %. The flow cytometry showed that after incubated with the leaf of fast-growing eucylyptus extract for diluted 30 times, the early of apoptosis were 30.03%. The ratios of apoptotic increased with concentration diluted times decreased, and it was in a time-dependent manner. Moreover, the ratios of apoptotic increased with the time prolonged of the leaf of fast-growing eucylyptus extract, and it was in a dose-dependent manner. Conclusion: The leaf of fast-growing eucylyptus extract can inhibit proliferation and induced apoptosis on HepG2 Cells in a time-and dose-dependent manner. |
查看全文
查看/发表评论 下载PDF阅读器 |
关闭 |