| 孙丽艳,陶萍萍,杨宾烈,原杰彦,王宇华,张 蕾,张 爱,余 雯,李 芳.宫颈HPV16持续感染阶段宫颈P16和Ki67的表达及其与宫颈癌变的关系[J].,2018,(21):4103-4106 |
| 宫颈HPV16持续感染阶段宫颈P16和Ki67的表达及其与宫颈癌变的关系 |
| Expression of P16/Ki67 in Patients with Cervical HPV16 Persistent Infection and Its Correlation with the Incidence of Cervical Cancer |
| 投稿时间：2018-02-25 修订日期：2018-03-21 |
| DOI：10.13241/j.cnki.pmb.2018.21.022 |
| 中文关键词: HPV16 宫颈癌 持续感染 P16 Ki67 转归 |
| 英文关键词: HPV16 Cervical cancer Persistent infection P16 Ki67 Outcome |
| 基金项目:上海市浦东新区人民医院科技项目(PRKT2014-08)；上海市浦东新区卫生系统重点专科项目(PWZz2013-14) |
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| 中文摘要: |
| 摘要 目的：探讨宫颈人乳头状瘤病毒(HPV)16持续感染阶段宫颈P16和Ki67的表达及其与宫颈癌变的相关性。方法：采用P16/Ki67免疫组化双染法检测102例HPV16持续感染者、136例非持续感染者宫颈组织P16、Ki67蛋白的表达，并根据免疫组化结果分组为双染阳性组、双染阴性组。所有患者随访观察2年，比较两组患者的结局及宫颈癌前病变的发生率。结果：P16、Ki67及P16/Ki67双染的阳性率分别为40.3%、44.5%及34.0%，HPV16持续感染患者P16、Ki67及P16/Ki67双染的阳性率均显著高于非持续感染患者(P<0.05)。HPV16持续感染患者的P16、Ki67蛋白表达呈显著正相关(P<0.05)。HPV16持续感染患者中，双染阳性组的病情持续和进展比例明显高于双染阴性组，也明显高于HPV16非持续感染(双染阴性组、双染阳性组)患者(P<0.05)。HPV16持续感染患者中，双染阳性组进展为HSIL及以上病变发生率为32.5%(13/40)，显著高于双染阴性组6.5%(P<0.05)。结论：P16，Ki67双染阳性在HPV16持续感染阶段与宫颈上皮内病变疾病进展成正相关，对HPV16持续感染进展为宫颈高度病变有预警价值，可作为HPV16阳性早期治疗的敏感指标。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the expression of cervical P16/Ki67 in patients with cervical human papillomavirus 16(HPV16) persistent infection and its correlation with the incidence of cervical cancer. Methods: P16/Ki-67 dual immunostaining was performed to detect the expression of P16/Ki67 in 102 cases of patients with cervical HPV16 persistent infection,136 patients with cervical HPV16 non-persistent infection, all the patients were divided into dual stain positive group and dual stain negative group according to the im- munohistochemical results. All the patients were followed up for 2 years, the difference in the outcome and early-stage cervical cancer and incidence of cervical cancer were compared between the two groups. Results: The positive rate of P16, Ki67 and P16/Ki67 dual stain were 40.3%, 44.5% and 34.0% respectively. The positive rate of P16, Ki67 and P16/Ki67 dual stain in HPV16 persistent infection pa- tients was significantly higher than that in non-persistent infection patients(P<0.05). P16, Ki67 were significantly and positively correlat- ed in persistent infection patients (P<0.05). The outcome in dual stain negative group was superior to that in dual stain positive group (P<0.05). In HPV16 persistent infection patients, the outcome in dual stain positive group was inferior to that in dual stain negative group, and inferior to that in dual stain negative and positive group of persistent infection patients, the proportion of advance patients in- creased significantly (P<0.05). In HPV16 persistent infection patients, the progression e towards cervical HSIL in dual stain positive group was 32.5%, which was significantly higher than that in dual stain negative group that was 6.5% (P<0.05). Conclusion: P16 and Ki67 dual-stain positive were correlated with the progression of cervical intraepithelial neoplasia in the stage of HPV16 persistent infec- tion. It has an early warning value for the progression of HPV16 persistent infection to cervical high-grade lesions. It can be used as a sensitive indicator of HPV16 positive early treatment. |
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