| 周 琳,卫秦川,王豫椒,徐美翔,熊文东,刘 霞,任勇刚.NFE2L1在食管鳞癌中的表达及临床意义[J].,2018,(19):3706-3711 |
| NFE2L1在食管鳞癌中的表达及临床意义 |
| Expression and Clinical Significance of NFE2L1 in Esophageal Squamous Cell Carcinoma |
| 投稿时间：2018-04-11 修订日期：2018-05-09 |
| DOI：10.13241/j.cnki.pmb.2018.19.024 |
| 中文关键词: 转录因子NFE2L1 食管鳞癌 免疫组化 生物信息 |
| 英文关键词: Transcription factor NFE2L1 Esophageal Squamous Cell Carcinoma Immunohistochemistry Bioinformatics |
| 基金项目:四川省科技计划项目（2017JY0130）；川北医学院博士启动基金项目（CBY16-QD07）；四川省大学生创新创业训练计划项目（201710634002） |
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| 中文摘要: |
| 摘要 目的：通过检测食管鳞癌标本中核因子E2相关因子1（NFE2L1）的表达情况，探究NFE2L1在食管鳞癌发生发展中的作用，为食管鳞癌的诊治以及预后评估提供新的思路。方法：收集我校附院2016-2017年经病理组织学检查诊断为食管鳞癌的手术标本40例及其对应的癌旁组织并从NCBI数据库下载GEO测序数据，应用定量PCR、免疫组化和生物信息分析等方法检测分析NFE2L1基因的表达情况。结果：在食管鳞癌组织中，NFE2L1表达阳性31例（77.5%），癌旁组织阳性表达17例（42.5%），两组比较差异显著有统计学意义（P＜0.001）；进一步发现NFE2L1的阳性表达与肿瘤分化程度和淋巴转移相关（P＜0.05）。但在不同年龄、性别、浸润深度及不同部位之间差异无统计学意义。GEO数据分析结果显示NFE2L1在食管鳞癌组织显著高表达（P＜0.01），只是未达到显著差异表达的阈值标准（即变化倍数小于2倍）。结论：NFE2L1在食管鳞癌中高表达，表达的高低与食管鳞癌的发生进展密切相关。 |
| 英文摘要: |
| ABSTRACT Objective: This article aims to explore the role of NFE2L1 in the development of esophageal squamous cell carcinoma and offer a new idea for the treatment and prognosis evaluation of esophageal squamous cell carcinoma. Methods: Through collecting 40 cases of surgical specimens which were diagnosed as esophageal squamous cell carcinoma and its corresponding para-cancerous tissues in NSMC's affiliated hospital from 2016 to 2017 and downloading GEO sequencing data from the NCBI database, the expression of NFE2L1 will be analyzed from quantitative PCR, immunohistochemistry and bioinformatics analysis. Results: NFE2L1 expression was positive in 31 esophageal cancer tissues(77.5%) and 17 paracancerous tissues (42.5%), the remarkable difference between the two groups was statistically significant(P<0.001). This article revealed further that NFE2L1's positive expression was correlated with the degree differentiation of Tumor Cell and the transfer of lymphatic metastasis(P<0.05). However, there was no statistic significance in different age, gender, depth of infiltration and different parts. GEO analysis showed that NFE2L1 was highly expressed in esophageal squamous cell carcinoma(P<0.01), but it did not reach the threshold criterion of significant difference(i.e. the change multiple was less than 2 times). Conclusion: NFE2L1 is highly-expressed in esophageal squamous cell carcinoma and its expression is closely related to the occurrence and development of esophageal cancer. |
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