| 徐震超,王锡阳,刘 政,陈巩舟,陈 刚.IFN-γ、IL-12及TNF-α在脊柱结核病人血清和病灶中的表达以及对疾病的影响[J].,2018,(16):3057-3061 |
| IFN-γ、IL-12及TNF-α在脊柱结核病人血清和病灶中的表达以及对疾病的影响 |
| The Expression of IFN-γ, IL-12 and TNF-α in the Serum and Lesions of Spinal Tuberculosis and Its Effects on the Disease |
| 投稿时间：2017-10-29 修订日期：2017-11-25 |
| DOI：10.13241/j.cnki.pmb.2018.16.012 |
| 中文关键词: 脊柱结核 干扰素-γ 白细胞介素-12 肿瘤坏死因子-α |
| 英文关键词: Spinal tuberculosis Interferon-γ Interleukin-12 Tumor necrosis factor-α |
| 基金项目:国家自然科学基金项目（81672191） |
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| 中文摘要: |
| 摘要 目的：检测IFN-γ、IL-12及TNF-α在脊柱结核病人血清和病灶中的表达水平，探讨血清和病灶中IFN-γ、IL-12及TNF-α的表达水平与脊柱结核的相关性。方法：2014年12月至2016年3月期间，纳入符合标准的脊柱结核病人46例为实验组，椎体骨折病人48例为对照组。应用ELISA检测两组人群血清中IFN-γ、IL-12、TNF-α的平均浓度；病灶标本免疫组化染色检测其IFN-γ、IL-12、TNF-α的平均光密度值。结果：实验组血清中IFN-γ, IL-12, TNF-α平均浓度分别为26.82±7.81 pg/mL，25.93±12.84 pg/mL，68.13±16.24 pg/mL，对照组血清中平均浓度分别为46.89±7.81 pg/mL，49.45±22.38 pg/mL，26.65±10.11 pg/mL；实验组病灶中IFN-γ, IL-12, TNF-α平均光密度值分别为0.273±0.076，0.380±0.054，0.306±0.052，对照组病灶中平均光密度值分别为0.092±0.024，0.183±0.034，0.180±0.031。实验组与对照组血清及病灶中IFN-γ、IL-12及TNF-α的表达水平比较，差异有统计学意义(P<0.05)。结论：脊柱结核病人的血清IFN-γ、IL-12浓度呈低表达，TNF-α浓度呈高表达；脊柱结核病人病灶中IFN-γ、IL-12及TNF-α表达增加；检测脊柱结核病人IFN-γ、IL-12及TNF-α血清水平含量能为其机体免疫力的状态、判断结核病情程度和进展及为临床诊断提供参考依据。 |
| 英文摘要: |
| ABSTRACT Objective: To detect the serum and lesions levels of IFN-γ, IL-12 and TNF-α expressed in spinal tuberculosis patients change, and to preliminary explore the association between IFN-γ, IL-12 and TNF-α levels and spinal tuberculosis. Methods: 46 patients with spinal tuberculosis as the experimental group, 48 cases of vertebral fracture were recruited as the control group in our study from December 2014 to March 2016. The concentration of serum IFN-γ, IL-12 and TNF-α were detected by ELISA. IFN-γ, IL-12 and TNF-α immunohistochemical staining were performed on the lesions, the average optical density values of IFN-γ, IL-12 and TNF-α were detected. Results: The mean concentrations of IFN-γ, IL-12 and TNF-α in serum of experimental group were 26.82±7.81 pg/mL, 25.93±12.84 pg/mL, 68.13±16.24 pg/mL respectively. The mean concentrations of IFN-γ, IL-12 and TNF-α in serum of control group were 46.89±7.81 pg/mL, 49.45±22.38 pg/mL, 26.65±10.11 pg/mL respectively. The average optical density of IFN-γ, IL-12 and TNF-α in the experimental group was 0.273±0.076, 0.380±0.054, 0.306±0.052 respectively. The average optical density of IFN-γ, IL-12 and TNF-α in the control group was 0.092±0.024, 0.183±0.034, 0.180±0.031 respectively. The mean serum IFN-γ and IL-12 concentration in experimental group was significantly lower than control group (P<0.05); the mean serum TNF-α concentration in experimental group was significantly higher than control group (P<0.05). The IFN-γ, IL-12 and TNF-α mean optical density of lesions in experimental group was significantly higher than control group (P<0.05). Conclusion: Serum IFN-γ, IL-12 concentrations of spinal tuberculosis patients are lower than normal, serum TNF-α concentration of spinal tuberculosis patients are higher than normal; High expression of IFN-γ, IL-12 and TNF-α in lesion of spinal tuberculosis; Detecting the serum IFN-γ, IL-12 and TNF-α concentration of patients able to be provide a basis for the state of immunity, the severity and progress of tuberculosis and the clinical diagnosis. |
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