| 王 宇,宋 舟,康爱文,赵 蒙,田丽媛.白花蛇舌草提取物对乳腺癌MCF-7细胞增殖和凋亡影响的实验研究[J].,2018,(16):3007-3011 |
| 白花蛇舌草提取物对乳腺癌MCF-7细胞增殖和凋亡影响的实验研究 |
| An Experimental Study on the Effects of Hedyotis Diffusa Extracts on the Proliferation and Apoptosis of MCF-7 Breast Cancer Cells |
| 投稿时间：2017-10-31 修订日期：2017-11-23 |
| DOI：10.13241/j.cnki.pmb.2018.16.002 |
| 中文关键词: 白花蛇舌草 乳腺癌 细胞增殖 MTT检测 细胞凋亡 |
| 英文关键词: Hedyotis diffusa Breast cancer Cell proliferation MTT assay Cell apoptosis |
| 基金项目:国家自然科学基金青年基金项目(81201336)；三亚市医疗卫生科技创新项目(2015YW21) |
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| 中文摘要: |
| 摘要 目的：探索不同浓度的白花蛇舌草提取物对人乳腺癌MCF-7细胞的增殖和凋亡的影响。方法：选择体外培养的人乳腺癌MCF-7细胞，将其分为5组，分别给予不同浓度使用乙醇提取法提取的白花蛇舌草有效成分(0、75、150、300和600 μg/mL)。分别在药物孵育的第1、2、3、4天提取细胞样本，采用MTT法检测细胞的增殖情况，流式细胞技术检测细胞周期和细胞凋亡情况。结果：在第1、2、3和4天，不同浓度的白花蛇舌草均能够显著抑制乳腺癌细胞的增殖，且细胞的增殖抑制率随着药物浓度的升高而升高，以300 μg/mL和600 μg/mL两个浓度的作用效果最为显著，但300 μg/mL和600 μg/mL两个浓度间无统计学差异(P>0.05)。不同浓度的白花蛇舌草干预后处于G0~G1期的细胞百分比显著低于对照组(P<0.05)，处于S期和G2~M期的细胞百分比高于对照组(P<0.05)，且细胞凋亡率显著高于对照组(P<0.05)，对细胞周期的调控和凋亡的抑制效果以300 μg/mL和600 μg/mL两个浓度最为显著，但两个浓度间无统计学差异(P>0.05)。结论：白花蛇舌草能够显著抑制乳腺癌MCF-7细胞的增殖并促进细胞凋亡，且这一作用存在显著的“量-效”关系。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effects of various concentrations of hedyotis diffusa extracts on the proliferation and apoptosis of MCF-7 human breast cancer cells. Methods: The MCF-7 human breast cancer cells were cultured in the DMEM medium with 10% fetal bovine serum, 1% penicillin/streptomycin and insulin. The active components of hedyotis diffusa were extracted using the ethanol-extracting method. Overall 5 groups were included in the study, including four hedyotis diffusa administration groups with different concentrations (75, 150, 300 and 600 μg/mL) and the blank control group. Results: The cell samples were collected at 1, 2, 3, and 4 days post the drug application, and the effects of hedyotis diffusa on cellular proliferation rate were evaluated the MTT and CCK-8 methods, respectively. Then, the efficacy of hedyotis diffusa with different concentrations (75, 150, 300 and 600 μg/mL) on the cell cycle and cell apoptosis was investigated based on the flow cytometry technique. Conclusion: Hedyotis diffusa could significantly inhibit the proliferation and promote the apoptosis of breast cancer cells in a concentration dependent manner. |
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