| 张玉蓉,高荣敏,陈家艺,杨 光,向 薇.青蒿琥酯对肺癌细胞裸鼠皮下移植瘤生长和放射敏感性的影响研究[J].,2018,(15):2861-2865 |
| 青蒿琥酯对肺癌细胞裸鼠皮下移植瘤生长和放射敏感性的影响研究 |
| Effect of Artesunate on the Growth and Radiosensitivity of Lung Cancer Cells Subcutaneously Transplanted in Nude Mice |
| 投稿时间：2017-12-14 修订日期：2018-01-19 |
| DOI：10.13241/j.cnki.pmb.2018.15.012 |
| 中文关键词: 青蒿琥酯 肺癌 皮下移植瘤 放射敏感性 |
| 英文关键词: Artesunate Lung cancer Subcutaneous tumor Radiosensitivity |
| 基金项目:湖北省卫生和计划生育委员会科研项目(WJ2013MB328) |
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| 中文摘要: |
| 摘要 目的：探讨青蒿琥酯对肺癌细胞裸鼠皮下移植瘤生长和放射敏感性的影响。方法：将肺癌细胞接种至裸鼠皮下，腹腔注射青蒿琥酯，同时给予放射处理记为联合组，设置青蒿琥酯组(不照射处理)、放射组(腹腔注射生理盐水)和对照组(腹腔注射生理盐水，不放射处理)，测量肿瘤体积，取瘤体并称取瘤体重量，脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)法检测组织中细胞凋亡水平，Western blot检测组织中活化的含半胱氨酸的天冬氨酸蛋白水解酶3(Cleaved Caspase-3)、信号转导与转录因子3(STAT3)、磷酸化的STAT3(p-STAT3)、p38丝裂原活化蛋白激酶(p38MAPK)、磷酸化的p38MAPK(p-p38MAPK)水平。结果：青蒿琥酯组、放射组、联合组皮下移植瘤体积和重量均明显低于对照组，联合组肿瘤重量和体积明显低于青蒿琥酯和放射组，差异均具有统计学意义(P<0.05)。青蒿琥酯、放射组、联合组肿瘤组织中细胞凋亡率、Cleaved Caspase-3水平和p-p38MAPK/p38MAPK均明显高于对照组，联合组肿瘤组织中细胞凋亡率、Cleaved Caspase-3水平和p-p38MAPK/p38MAPK高于青蒿琥酯和放射组，差异均具有统计学意义(P<0.05)。青蒿琥酯、放射组、联合组肿瘤组织中p-STAT3/STAT3水平明显低于对照组，联合组肿瘤组织中p-STAT3/STAT3水平低于蒿琥酯组和放射组，差异均具有统计学意义(P<0.05)。结论：青蒿琥酯能够抑制肺癌细胞裸鼠皮下移植瘤生长，促进癌细胞凋亡，增加放疗敏感性，作用机制可能与p38MAPK、STAT3信号通路有关。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of artesunate on the growth and radiosensitivity of lung cancer cells subcutaneously transplanted subcutaneously in nude mice. Methods: Lung cancer cells were inoculated subcutaneously in nude mice, intraperitoneal in- jection of artesunate, the radiation treatment was also recorded as a combined group, set the artesunate group at the same time (not irradi- ation), radiation group (intraperitoneal injection of normal saline) and control group (intraperitoneal injection of saline, no radiation treat- ment), measurement of tumor volume, tumor tissues were collected and weighed tumor weight, TUNEL method was used to detect the apoptotic level in the tissues, the levels of Cleaved Caspase-3, STAT3, p-STAT3, p38MAPK and p-p38MAPK in tissues were detected by Western and blot. Results: Artesunate group, radiotherapy group and combined group subcutaneous transplantation tumor volume and weight were significantly lower than the control group, the combined group of tumor weight and volume was significantly lower than that of artesunate group and radiation group, the differences were statistically significant (P<0.05). Artesunate group, radiotherapy group and combined group tumor cell apoptosis rate and level of Cleaved Caspase-3 and p-p38MAPK/p38MAPK were significantly higher than the control group, combination group tumor cell apoptosis rate, the Cleaved Caspase-3 level and p-p38MAPK/p38MAPK level were higher than that of artesunate group and radiation group, the differences were statistically significant (P<0.05). Artesunate group, radiotherapy group and combined group p-STAT3/STAT3 in tumor tissues was significantly lower than the control group, the level of p-STAT3/STAT3 in tumor tissue in the combined group was significantly lower than artesunate group and radiation group, the differ- ences were statistically significant (P<0.05). Conclusion: Artesunate can inhibit the growth of lung cancer cells subcutaneously trans- planted in nude mice, promoting apoptosis of cancer cells, increased radiosensitivity, the mechanism of action may be related to p38MAPK and STAT3 signaling pathways. |
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