| 孟 丹,邱越佳,杨婷媛,王连艳,王 硕,朱艳华,马光辉.聚合物脂质纳米球的制备优化[J].,2018,(10):1887-1891 |
| 聚合物脂质纳米球的制备优化 |
| Preparation and Characterization of Polymer-lipid Nanoparticles |
| 投稿时间：2017-10-30 修订日期：2017-12-06 |
| DOI：10.13241/j.cnki.pmb.2018.10.016 |
| 中文关键词: 脂质纳米球 超声复乳法 响应面法 蛋白类药物 |
| 英文关键词: Polymer-lipid nanoparticles Ultrasonic double emulsion method Response surface methodology Protein drugs |
| 基金项目:国家自然科学基金项目（21476243） |
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| 中文摘要: |
| 摘要 目的：制备粒径均一且稳定、载药率和包埋率较高的聚合物脂质纳米球。方法：将HSPC（氢化大豆卵磷脂）与PLGA（聚乳酸-羟基乙酸共聚物）两种材料结合，利用超声复乳法制备聚合物脂质纳米球，采用响应面法优化最佳制备工艺；以HSPC（氢化大豆卵磷脂）与PLGA（聚乳酸-羟基乙酸共聚物）的比例、PVA浓度、超声功率为条件优化制备参数，以粒径为响应值。结果：优化后的最佳工艺参数为：HSPC与PLGA的比例为1:10，PVA浓度为0.66%，超声功率为51.34% （205.36 W）。结论：按最优工艺制备出的聚合物脂质纳米粒的粒径为230 nm左右，多分散系数（PDI）值为0.057，与预测值偏差较小，且粒径分布均一，可作为蛋白及多肽类药物的递送载体。 |
| 英文摘要: |
| ABSTRACT Objective: In order to prepare stable polymer-lipid nanoparticles with uniform particle size, high drug loading and en- capsulation efficiency. Methods: HSPC (Hydrogenated Soybean Phospholipids Mixed Compounds) and PLGA (Poly(lactic-co-glycolic acid) ) were combined to prepare the polymer-lipid nanoparticles with ultrasonic double emulsion method. The preparation process was optimized using Response Surface Methodology（RSM）. The ratios of HSPC-PLGA, PVA concentrations and ultrasonic power were em- ployed as three main factors affecting the formation of nanoparticles in the preparation process. The particle size was chose as the re- sponse value. Results: The optimal conditions were obtained as follows: the ratio of HSPC-PLGA was 1:10, the concentration of PVA was 0.66%, and the ultrasonic power was 51.34% (205.36 W) respectively. Conclusion: The size of nanoparticles prepared under the op- timal conditions was about 230 nm with narrow size distributions, and the PDI value was 0.057, which was closer to the predicted value. The established polymer-lipid nanoparticles could be used as protein and polypeptide drugs in delivery system. |
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