| 邵景立,曹志强,高国梁,张启福,张 伟,李胜学,王丰梅.高海拔地区家兔挤压综合征模型的初步实验研究[J].,2018,(8):1463-1468 |
| 高海拔地区家兔挤压综合征模型的初步实验研究 |
| Preliminary Experimental Study on A Model of Rabbits Crush Syndrome in High Altitude |
| 投稿时间：2017-08-25 修订日期：2017-09-22 |
| DOI：10.13241/j.cnki.pmb.2018.08.012 |
| 中文关键词: 高原 挤压综合征 动物模型 家兔 |
| 英文关键词: Plateau Crush Syndrome Animal model Rabbits |
| 基金项目:青海省科技厅科技创新能力促进计划项目（2014-ZJ-746） |
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| 中文摘要: |
| 摘要 目的：建立一种适合高海拔地区的、简单有效并且可复制的家兔挤压综合征（crush syndrome，CS）实验模型，为在高原地区进一步研究CS打下基础。方法：健康成年新西兰家兔50只（饲养和实验地为西宁，海拔2260 m）。随机分为1组对照组及9个实验组，按照挤压重量（20 kg、30 kg、40 kg）和挤压时间（4 h、6 h、8 h）的不同进行排列组合后形成9种不同的挤压条件，实验组于解除挤压后6 h取静脉血检测血BUN、Cr、AST、ALT、CK、K+，解除压迫后24小时取家兔心肌、肾脏、肝脏、挤压处大腿四头肌行病理检查。对照组5只家兔作为空白对照，仅作麻醉处理不做挤压，在相同的时间点上取血及组织标本。结果：所有实验组家兔双后肢出现了明显的肿胀（P＜0.05）；所有实验组家兔在解压后6 h检测血BUN、Cr、AST、ALT、CK变化与对照组相比较均有统计学意义(p＜0.05)，而且随着压迫重量和压迫时间的增加，各数值也随之增加；受压肌肉及肝肾组织活检提示均有显著的病理改变；但是随着压迫重量和时间的增加，实验组家兔死亡率也随之升高。结论：随着挤压重量和挤压时间的增加可加重挤压综合征的严重程度，在高海拔地区，为了保证较低死亡率可采取挤压双后肢、挤压重量为20 Kg、挤压时间为4 h作为高海拔地区挤压综合征家兔模型的实验条件。 |
| 英文摘要: |
| ABSTRACT Objective: To establish a simple,effective and reproducible the rabbit model of Crush Syndrome(CS) in high altitude area, and to lay the foundation for further research on CS in the plateau region. Methods: A total of 50 healthy adult New Zealand rabbits, feeding and experimenting in Xi Ning (altitude is 2260), were randomly divided into 1 control group and 9 experimental groups. The experimental groups based on 9 kinds of combination with different levels of compression weight(20, 30, 40 Kg), time(4, 6, 8 hours).Serum BUN, Cr, ALT, AST, CK, K+ were measured six hours after decompression. Muscles, livers, myocardia and kidneys were evaluated morphologically. Control group rabbits were used as blank control, the blood and tissue samples were taken at the same time. Results: In all the experimental groups, there were obvious swelling in both hind limbs(p＜0.05). All the serum BUN, Cr, ALT, AST, CK were changed significantly in the 6 hours after decompression compared with control group, and increased with the increase and prolongation of the compression weight and time. The pathological changes of the muscle, liver and kidney were obvious. But with the increase of the compression weight and time, the mortality of the experimental group increased gradually. Conclusion: The severity of crush syndrome rabbits can be increased with the increase of compression weight and time.In the high altitude area, in order to ensure that the lower mortality rate can be taken to squeeze the two hind legs, compression weight of 20 kg, compression time for 4 hours as a high altitude crush syndrome rabbit model of experimental conditions. |
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