| 张 凡,李卫红,李 峰,刘 燕,于 雪,王 旭,高誉珊,胡艳红,张炜悦,孙天石,张楚焌,董一昕.天香胶囊对晕动病模型大鼠行为学与前庭核c-fos蛋白表达的影响[J].,2018,(5):807-811 |
| 天香胶囊对晕动病模型大鼠行为学与前庭核c-fos蛋白表达的影响 |
| Effects of Tianxiang Capsule on the Behavior and the Expression of c-fos in the Vestibule Nucleus of Rats with Motion Sicknes |
| 投稿时间：2017-10-21 修订日期：2017-11-08 |
| DOI：10.13241/j.cnki.pmb.2018.05.002 |
| 中文关键词: 天香胶囊 晕动病 行为学 c-fos蛋白 |
| 英文关键词: Tianxiang capsule Motion sickness Behavioristics c-fos |
| 基金项目:国家自然科学基金项目（81273885）；北京中医药大学"航海中医药"协同创新项目（522/0100604299）；北京中医药大学"重点学科"开放课题--"航海中医药学"（522/0100604054） |
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| 中文摘要: |
| 摘要 目的：观察中药复方天香胶囊对晕动病模型大鼠行为学与前庭核c-fos蛋白表达的影响。方法：选择雄性SD大鼠并将其随机分为正常组、模型组、阳性药组、天香胶囊低、中、高剂量组。灌胃预给药3天后，采用双轴旋转刺激法建立大鼠晕动病模型，通过晕反应指数评分、自发活动实验、异嗜高岭土实验检测各组大鼠行为学变化，Western blotting法与免疫组化法检测各组大鼠前庭核c-fos蛋白的表达。结果：与正常组大鼠相比，模型组大鼠晕反应指数和高岭土摄入量均显著增高，自发活动总路程与次数均显著减少，前庭核c-fos蛋白表达水平显著增高；与模型组相比，天香胶囊可显著降低晕动病模型大鼠晕反应指数与高岭土摄入量，提高自发活动总路程与活动次数，降低大鼠前庭核组织c-fos蛋白表达。结论：天香胶囊可有效改善晕动病模型大鼠的行为学变化，其作用机制可能与下调前庭核c-fos表达有关，提示降低前庭核兴奋性可能是天香胶囊抗晕动病的内在机制之一。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of Tianxiang capsule on the behavior and c-fos expression in the vestibule nucleus of rats with motion sickness. Methods: Male SD rats were randomly divided into six groups, including normal group, model group, scopolamine group, low-dose, mid-dose and high-dose Tianxiang capsule groups. Prior to biaxial rotation stimulus inducing rats motion sickness model, the drugs were intragastrically administrated for 3 days. The behavioral changes of rats were evaluated by the motion sickness index, the spontaneous activity test and Kaolin pica. Meanwhile, the expression of c-fos was detected by Western Blotting and Immunohistochemistry. Results: Compared with the normal group, the motion sickness index and Kaolin intake were increased, total traveling distance and frequency were decreased, the c-fos expression was up-regulated significantly in the model group. Compared with the model group, the motion sickness index and Kaolin intake were decreased, total traveling distance and frequency were increased, the c-fos expression was down-regulated significantly in Tianxiang capsule groups. Conclusion: Tianxiang capsule could effectively im- prove the behavioral changes of rats with motion sickness, which might be related to the down-regulation of c-fos in vestibular nucleus. Inhibiting the excitability of vestibular nucleus may be the pharmacological mechanisms of Tianxiang capsule in motion sickness. |
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