| 曹 薇,曹金军,刘江伟,李佳佳,沈才福,宋来阳,马 娜,是文辉,许 琴.姜黄素预处理对沙漠干热环境热射病大鼠肺损伤及HMGB-1和ICAM-1 mRNA的影响[J].,2018,(4):652-656 |
| 姜黄素预处理对沙漠干热环境热射病大鼠肺损伤及HMGB-1和ICAM-1 mRNA的影响 |
| Effects of Curcumin Pretreatment on the Lung Injury and the Expressions of HMGB-1 and ICAM-1 mRNA of Rats Induced by Heatstroke in Dry-heat Environment |
| 投稿时间：2017-09-06 修订日期：2017-09-27 |
| DOI：10.13241/j.cnki.pmb.2018.04.011 |
| 中文关键词: 姜黄素 干热环境 热射病 肺损伤 高迁移率族蛋白1 细胞间黏附分子-1 |
| 英文关键词: Curcumin Dry-heat environment Lung injury Heatstroke High mobility group box 1(HMGB1) Intercellular cell adhesion molecule-1(ICAM-1) |
| 基金项目:新疆自治区自然科学基金面上项目（2015211C231） |
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| 中文摘要: |
| 摘要 目的：研究姜黄素预处理对干热环境热射病大鼠肺损伤的保护作用及可能机制。方法：将50只SD大鼠随机分为5组(n=10)：常温对照组(NC)、干热对照组(DHC)、低剂量姜黄素预处理组(50 mg/kg)，中剂量姜黄素预处理组(100 mg/kg)及高剂量姜黄素预处理组(200 mg/kg)。NC、DHC组给予生理盐水灌胃，姜黄素预处理组给予不同剂量的姜黄素灌胃，每天1次，连续7天。第8天除NC组外，其余4组大鼠转移至西北特殊环境人工实验舱内进行实验，环境温度(41±0.5)℃，湿度(10±1)%。实验的第150分钟达到热射病状态，麻醉后取材。大鼠肺组织通过HE染色并进行肺损伤病理学评分，并应用RT-PCR检测肺组织HMGB1 mRNA和ICAM-1 mRNA的表达。结果：干热对照组大鼠肺组织病理评分、肺组织HMGB1和ICAM-1 mRNA表达较常温对照组、中、高剂量姜黄素预处理组均显著升高(P<0.01)，高剂量姜黄素预处理组大鼠肺组织病理评分明显低于低、中剂量姜黄素预处理组(P<0.01)，肺组织HMGB1和ICAM-1 mRNA表达在高剂量姜黄素预处理组明显低于低剂量姜黄素预处理组(P<0.01)。肺组织HMGB1和ICAM-1 mRNA的表达均与肺损伤评分具有显著正相关性(r=0.629、0.689，P<0.01)，HMGB1 mRNA和ICAM-1 mRNA之间的表达也呈显著正相关性(r=0.437，P<0.01)。结论：姜黄素可能部分通过抑制HMGB1表达的上调，减少下游的ICAM-1的表达来减轻炎症反应，从而发挥肺损伤保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the protective effects of curcumin pretreatment on the lung injury of heatstroke rats in dry-heat environment of desert and the underlying mechanisms. Methods: 50 Sprague-Dawley rats were randomly divided into five groups (n=10): normal control group(NC), dry heat control group(DHC), low dose curcumin pretreatment group (50 mg/kg), middle dose curcumin pretreatment group(100 mg/kg), high dose curcumin pretreatment group(200 mg/kg). The NC and DHC group were given a gavage of normal saline, while curcumin pretreatment group were given a gavage of curcumin with the concentration of 50 mg/kg, 100 mg/kg, 200 mg/kg once a day for 7 consecutive days. On the 8th day, all except for NC group were transferred to the climate cabin (The Simulated Climate Cabin for Special Environment of Northwest of China) with the conditions of (41±0.5)℃ temperature, (10±1) % relative humidity. At the time of 150th minute since the experiment began, the rats were in heatstroke states, the same were done to Control group. Lung tissues were harvested and pathological changes were observed by HE staining, HMGB1 and ICAM-1 mRNA were detected by real-time polymerase chain reaction(PCR). Results: The pathological lung injury score, the expressions of HMGB1 and ICAM-1 mRNA in lung tissues were signifcantly higher in the dry heat control group than those of the normal control group, middle dose and high dose curcumin pretreatment groups (P<0.01); the lung pathological injury scores were signifcantly lower in high dose curcumin pretreatment group than that of low dose and middle dose curcumin pretreatment groups (P<0.01); the expressions of HMGB1 and ICAM-1 mRNA in lung tissues were significantly lower in high dose curcumin pretreatment group than that of low dose curcumin pretreatment group(P<0.01). Correlation analysis indicated that the expression of HMGB1 and ICAM-1 mRNA were positively correlated with lung injury scores(r=0.629, 0.689, P<0.01), the expression of HMGB1 mRNA and ICAM-1 mRNA were also positively correlated with each other(r=0.437, P<0.01). Conclusion: Curcumin may decrease the inflammtory reacion and then exert protective effects on lung injury partly through inhibiting the expression of HMGB1gene and its downstream gene ICAM-1. |
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