| 张 平,陈 睿,包综方,刘国峰,李 静,王 彦,阎 超.加压毛细管电色谱法测定复方芦丁片中芦丁和维生素C的含量[J].,2018,(3):447-452 |
| 加压毛细管电色谱法测定复方芦丁片中芦丁和维生素C的含量 |
| Determination of Rutin and Vitamin C in Compound Rutin Tablets by pCEC |
| 投稿时间：2017-05-28 修订日期：2017-06-22 |
| DOI：10.13241/j.cnki.pmb.2018.03.010 |
| 中文关键词: 芦丁 维生素C 加压毛细管电色谱 含量测定 |
| 英文关键词: Rutin Vitamin C Pressurized capillary electrochromatography Content |
| 基金项目:上海市科委科研计划项目（15142200200,16142200300）；上海市博士后基金项目（14R21422700）；上海市引进技术的吸收与创新专项（XC-ZXSJ-02-2016-11） |
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| 中文摘要: |
| 摘要 目的：采用加压毛细管电色谱法测定复方芦丁片中芦丁与维生素C的含量。方法：采用Trisep TM -2100加压毛细管电色谱仪，以C18毛细管色谱柱（300 mm×100 μm i.d.，1.8 μm）为固定相，流动相为甲醇-乙腈-0.4%磷酸（18:70:12），用三乙胺调节pH值至3.0，总流速为0.05 mL?min-1，检测波长为254 nm。结果：芦丁的线性范围为2.0～20 μg?mL-1（r2=0.999）；平均回收率为99.4%，RSD为1.2%（n=9）；维生素C的线性范围为2.0～50 μg?mL-1（r2=0.999）；平均回收率为99.3%，RSD为1.4%（n=9）。另外，本方法与原国标方法进行了比较，结果基本一致。结论：加压毛细管电色谱法可用于复方芦丁片有效成分含量的测定，该方法简单、快速、准确，为复方芦丁片质量控制提供了新检测技术。 |
| 英文摘要: |
| ABSTRACT Objective: A pressurized capillary electrochromatography (pCEC) method has been developed for determination of the active components in compound rutin tablets. Methods: The separation was carried out on a 300 mm×100 μm i.d. capillary packed with 1.8 μm ODS by using methanol-acetonitrile-0.4% phosphoric acid (18:70:12) as the mobile phase, and the pH was adjusted to 3.0 with triethylamine. The total flow rate was 0.05 mL?min-1. The UV absorption detection was carried out at 254 nm. Results: The linear ranges for rutin and vitamin C were 2.0~20 μg?mL-1 (r2=0.999) and 2.0~50 μg?mL-1 (r2=0.999), respectively. Mean recoveries of rutin and vi- tamin C were 99.4% with relative standard deviation 1.2% (n=9), and 99.3% with relative standard deviation 1.4% (n=9), respectively. Besides, the method was compared with the original GB method, the results were basically identical. Conclusion: The method can be used for the determination of compound rutin tablets, which is simple, rapid and accurate. It provides a new detection technology for the quality control of compound rutin tablets. |
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