| 王 玮,栾 晓,柳 洋,郭菲菲,孙向荣,徐 珞.Ghrelin、GHRP-6以及胃动素对大鼠胃肠运动影响研究[J].,2018,(3):428-432 |
| Ghrelin、GHRP-6以及胃动素对大鼠胃肠运动影响研究 |
| The Effects of Ghrelin, GHRP-6 and Motilin on the Gastroprokinetic in Rats |
| 投稿时间：2017-04-30 修订日期：2017-05-24 |
| DOI：10.13241/j.cnki.pmb.2018.03.006 |
| 中文关键词: ghrelin GHRP-6 胃动素 胃排空 小肠推进 |
| 英文关键词: Ghrelin GHRP-6 Motilin Gastric emptying Intestinal transit |
| 基金项目:国家自然科学基金项目（81470815；81270460；81500414）；山东省优秀中青年科学基金项目（BS2014YY009）；青岛市科技局项目（14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要 目的：探究ghrelin、GHRP-6以及胃动素对大鼠胃肠运动的影响。方法：在体实验观察ghrelin、GHRP-6及胃动素对大鼠胃排空及小肠推进的影响，离体实验观察ghrelin、GHRP-6及胃动素对大鼠电场刺激引起胃窦胃底平滑肌舒缩反应的影响，RT-PCR观察ghrelin mRNA在胃的表达。结果：在体研究发现ghrelin及GHRP-6能够促进胃排空及小肠推进，胃动素对胃排空及小肠推进无影响。离体研究发现，ghrelin 1 μM能够抑制胃底平滑肌4-8Hz开电刺激下的舒张反应，并增强胃底平滑肌1-8Hz及胃窦平滑肌4-16Hz断电刺激下的收缩反应；GHRP-6 1μM 能够抑制胃底平滑肌4-8Hz开电刺激下的舒张反应，并增强胃底平滑肌1-2Hz、8-16Hz及胃窦平滑肌4-8Hz断电刺激下的收缩反应；L-NAME增强ghrelin、GHRP-6诱导的胃底和胃窦平滑肌条舒缩作用。大鼠胃窦及胃底分布有ghrelin受体mRNA。结论：ghrelin和GHRP-6能够加快胃排空促进小肠推进，该效应可能是通过迷走神经通路及胆碱能兴奋产生的。 |
| 英文摘要: |
| ABSTRACT Objective: We compared the gastroprokinetic effects of ghrelin, the ghrelin receptor agonist, growth hormone releasing peptide 6 (GHRP-6), and motilin in rats in vivo and in vitro. Methods: In vitro observation the effect of ghrelin, GHRP-6 and motilin on rat gastric emptying and small intestinal propulsion, in vitro experimental observation the effect of ghrelin, GHRP-6 and motilin on the fundic or antral strips responses to EFS, RT-PCR observation the expression of ghrelin mRNA in the stomach. Results: Ghrelin and GHRP-6 but not motilin accelerated gastric emptying and transit in rats. 1 μM ghrelin can inhibit the on-relaxation of fundic strips(4-8Hz), and enhance off-response of fundic(1-8Hz) and antral strips(4-16Hz); 1 μM GHRP-6 can inhibit the on-relaxation of fundic strips(4-8Hz), and enhance off-response of fundic(1-2,8-16Hz) and antral strips(4-8Hz); the effect of ghrelin and GHRP-6 on fundic and antral strips can be enhance by L-NAME. The fundic and antral can express ghrelin mRNA. Conclusion: Ghrelin and GHRP-6 but not motilin accelerate gastric emptying and transit by activating cholinergic excitatory pathways in the enteric nervous system in addition to the known vagal pathways. |
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