| 杨 柳,郭海云,邵长健,朱凯龙,张伟东,才延辉,李 燕.糖原检测方法研究进展[J].,2018,(1):175-179 |
| 糖原检测方法研究进展 |
| Research Progress of Glycogen Detection Methods |
| 投稿时间：2017-02-27 修订日期：2017-03-21 |
| DOI：10.13241/j.cnki.pmb.2018.01.038 |
| 中文关键词: 糖原检测方法 PAS染色 糖原代谢 |
| 英文关键词: Glycogen detection methods Periodic acid schiff stain Glycogen metabolism |
| 基金项目:国家自然科学基金项目（81471110）；陕西省青年科技新星项目（2015KJXX-49）；中国博士后科学基金项目（2015T81094） |
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| 中文摘要: |
| 摘要：糖原是由葡萄糖分子通过糖苷键聚合而成的高分子物质，作为重要的能源物质储存于肝脏、肌肉和脑等重要器官。糖原的储存或代谢异常可引起多种疾病。一方面，作为一种动态能量底物，准确检测糖原的质与量有一定难度，另一方面，目前随着对糖原研究的深入，出现了越来越多的糖原检测方法和手段。因此，选择恰当的糖原检测方法以促进对糖原的研究显得尤为重要。本文对几种常用的糖原定性与定量检测方法的实验原理，操作步骤及影响因素和改进进行总结，比较其优缺点，为研究者选择最适合的方法对糖原代谢及相关疾病进行研究提供参考。 |
| 英文摘要: |
| ABSTRACT: Glycogen, a common macromolecular substance polymerized by glucose via glucosidic bond, is a significant energy source that is stored in liver, muscle, brain and other important organs. The abnormality of glycogen storage or metabolism can result in many diseases. On the one hand, as a kind of dynamic energy substrates, it is difficult to detect the quality and quantity of glycogen accu- rately. On the other hand, at present, with the thorough research of glycogen, there are more and more glycogen detection methods. So it is imperative to select appropriate glycogen detection methods to promote glycogen research. In this review, the experiment principle, op- eration procedures, influential factors and improvements of some common glycogen qualitative or quantitative detection methods are summarized. The advantages and disadvantages of each method are also compared, which could serve as a reference for researchers to se- lect optimal method to study glycogen metabolism and relevant diseases. |
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