| 王玉红,王 彦,阎 超.加压毛细管电色谱-微流蒸发光散射联用仪检测5种糖醇[J].,2018,(1):140-145 |
| 加压毛细管电色谱-微流蒸发光散射联用仪检测5种糖醇 |
| Determination of Five Sugar Alcohols by Pressurized Capillary Electrochromatography with Micro Flow Evaporative Light Scattering Detector |
| 投稿时间：2017-07-20 修订日期：2017-08-15 |
| DOI：10.13241/j.cnki.pmb.2018.01.031 |
| 中文关键词: 蒸发光散射检测器 加压毛细管电色谱 糖醇 |
| 英文关键词: Evaporative light scattering detector Pressurized capillary electrochromatography Sugar alcohol |
| 基金项目:国家重大科学仪器设备开发专项项目(2011YQ15007202)；上海市科委科研计划项目（16142200300, 17142201000）；中国博士后基金项目(2016M591702）；上海市引进技术的吸收与创新专项（XC-ZXSJ-02-2016-11） |
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| 中文摘要: |
| 摘要 目的：本论文使用加压毛细管电色谱-微流蒸发光散射（pCEC-μELSD）联用仪，建立了一种赤藓糖醇、木糖醇、山梨糖醇、麦芽糖醇和乳糖醇的分离检测方法。方法：实验采用亲水作用色谱柱，80%乙腈-水（含40 mmol/L三乙胺）为流动相，施加+5KV电压进行分离；蒸发光散射检测器蒸发温度120℃, 氮气载气流量0.8 L/min进行检测。结果：实验结果表明，5种糖醇在线性范围内线性关系良好，线性相关系数(r)均大于0.998；3个添加水平下的样品平均回收率在82.4%-107.2%之间，相对标准偏差RSD(n=3)小于4.0%；方法最低检出限（LOD, S/N=3）在4-11 ng之间。结论：该方法对比常规HPLC-ELSD法，分离速度快、重现性好、经济环保，具有较高的实用性，为无糖食品中糖醇的定量检测提供了一种快速且行之有效的方法。 |
| 英文摘要: |
| ABSTRACT Objective: A method for determination of five sugar alcohols (erythritol, xylitol, sorbitol, maltitol and lactitol) by pres- surized capillary electrochromatography with micro flow evaporative light scattering detector was established. Methods: The chromato- graphic separation was conducted on an hydrophilic column using 80% acetonitrile-water (containing 40 mmol/L triethylamine) as the mobile phase, with+5 kV applied voltage. The drift tube temperature of the ELSD was set at 120℃and the nitrogen flow rate was set at 0.8 L/min. Results: It was found that the reasonable linearity was achieved for five sugar alcohols with correlation coefficients no less than 0.998.The average recovery was between 82.4%-107.2% under three different level by using standard addition method, and the rela- tive standard deviation (RSD, n=3) was less than 4.0%. The limit of detection (LOD, S/N=3) was between 4 to 11 ng. Conclusion: Com- pared with the conventional HPLC-ELSD, the method has the advantages of high separation speed, good reproducibility, and environ- ment-friendly economy. The proposed method provides a fast and effective method for the quantitative detection of sugar alcohols in sug- ar-free foods. |
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