| 李凤蕾,周翠红,彭正午,薛姗姗.电针预处理对PTSD模型大鼠焦虑样行为及海马Sirt1/MAO-A基因表达的影响[J].,2017,17(35):6839-6841 |
| 电针预处理对PTSD模型大鼠焦虑样行为及海马Sirt1/MAO-A基因表达的影响 |
| The Influence of Electroacupuncture (EA) Preconditioning on Anxiety-like Behavior and the Expression of Sirt1/MAO-A in the Hippocampus of PTSD Rats |
| 投稿时间：2017-06-30 修订日期：2017-07-25 |
| DOI：10.13241/j.cnki.pmb.2017.35.009 |
| 中文关键词: PTSD 电针 Sirt1 MAO-A |
| 英文关键词: Post-traumatic stress disorder Electroacupuncture Sirt1 MAO-A |
| 基金项目:国家自然科学基金项目（81401109）；陕西省科技新星资助项目（2015KTXX-51） |
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| 中文摘要: |
| 摘要 目的：探讨电针预处理对创伤后应激障碍大鼠（PTSD）焦虑样行为的改善作用及对海马去乙酰化酶Sirt1及单胺氧化酶MAO-A基因表达的影响。方法：将32只SD大鼠随机分为对照组（Sham），模型组（PTSD），电针刺激组（EA），电针预处理+模型（EA + PTSD），每组8只。电针刺激组（EA）和电针预处理+模型（EA + PTSD）组大鼠每天给予百会穴电针刺激（2/15 Hz，1 mA）30 min，连续刺激7 d。然后对模型组（PTSD）组和电针预处理+模型（EA + PTSD）组大鼠进行ESPS造模处理。造模结束14天后，通过旷场和高架十字测试其焦虑样行为，随后处死大鼠，通过实时定量PCR（RT-PCR）检测海马Sirt1和MAO-A的mRNA表达情况。结果：（1）PTSD大鼠具有明显的焦虑样行为，包括旷场中心的探索减少（44.94±13.66 , 16.86±7.12, P<0.05）和高架十字开臂运动时间减少（55.90±26.39, 23.36±12.23, P<0.01），PTSD组大鼠海马Sirt1和MAO-A的mRNA表达增加（1.00±0.07, 1.90 ±0.05; 1.01±0.10, 1.54±0.15, P<0.01）。（2）电针预处理可以缓解PTSD大鼠的焦虑样行为，PTSD与EA + PTSD组之间存在显著性差异（16.86±7.12, 36.24±13.28, P<0.05; 23.36±12.23, 50.14±20.77, P<0.05）。（3）电针预处理可以抑制PTSD大鼠海马的Sirt1和MAO-A mRNA表达，PTSD与EA + PTSD组之间的Sirt1和MAO-A的mRNA水平存在显著性差异（1.90±0.05, 1.30±0.08, P<0.05; 1.54±0.15, 1.23±0.15, P<0.05）。结论：电针预处理可以调节PTSD大鼠海马的Sirt1和MAO-A的mRNA水平，缓解PTSD大鼠的焦虑样行为。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of Electroacupuncture (EA) preconditioning on anxiety-like behavior and the effect on hippocampus Sirt1/MAO-A expression of PTSD rats. Methods: SD rats were randomly assigned to the following 4 groups (n=8 for each group): Sham, PTSD, EA, EA + PTSD. The EA and EA + PTSD group received EA treatment at frequency of 2/15 Hz for 30 min daily for 7 days. Then, the PTSD and EA + PTSD group rats received SPS&S. After 14 days of treatment with SPS&S, rats were experi- enced open field test and elevated plus maze test, then sacrificed and the mRNA level of Sirt1 and MAO-A in the hippocampus were de- termined by real-time PCR. Results: (1) The rat in PTSD group showed significant less time in the central field（44.94±13.66 , 16.86±7.12, P<0.05）and decreased time in open arm（55.90±26.39, 23.36±12.23, P<0.01）, simultaneously mRNA level of Sirt1 and MAO-A in hippocampus were increased（1.00±0.07, 1.90±0.05; 1.01±0.10, 1.54±0.15, P<0.01）; (2) Electroacupuncture preconditioning re- versed anxiety behaviors in rats exposed to SPS&S paradigm（16.86±7.12, 36.24±13.28, P<0.05; 23.36±12.23, 50.14±20.77, P < 0.05）; (3) Electroacupuncture precondition restored the mRNA level of Sirt1 and MAO-A in the hippocampus of PTSD rat（1.90±0.05, 1.30±0.08, P<0.05; 1.54±0.15, 1.23±0.15, P<0.05）. Conclusion: Electroacupuncture preconditioning reversed anxiety behaviors in rats exposed to SPS&S paradigm and restored the mRNA level of Sirt1 and MAO-A in the hippocampus. |
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