| 周姝含,陈 冲,唐 敏,胡蓉蕾,李翠翠,唐 震.上调OPN和TIP30表达对口腔鳞癌cal-27细胞的影响[J].,2017,17(32):6209-6214 |
| 上调OPN和TIP30表达对口腔鳞癌cal-27细胞的影响 |
| The Influence of up-regulated Expressions of TIP30 and OPN on the Oral Squamous Cell Carcinoma Cal-27 Cells |
| 投稿时间：2017-05-04 修订日期：2017-05-27 |
| DOI：10.13241/j.cnki.pmb.2017.32.002 |
| 中文关键词: cal-27细胞株 OPN TIP30 增殖 迁移 |
| 英文关键词: Cal-27 cell line OPN TIP30 Proliferation Migration |
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| 中文摘要: |
| 摘要 目的：探讨上调骨桥蛋白(OPN)和TIP30表达对口腔鳞状细胞癌cal-27细胞增殖和迁移能力的影响。方法：采用过表达OPN载体病毒和过表达TIP30质粒分别感染或转染cal-27细胞株后，通过实时荧光定量PCR法和蛋白印迹western blot法检测OPN和TIP30的表达，CCK-8法检测细胞增殖能力，细胞划痕实验检测细胞的迁移能力。结果：过表达OPN载体病毒感染的cal-27-shOPN细胞株OPN mRNA和蛋白的表达均明显高于cal-27-shN细胞株(P<0.001，P=0.002)，且细胞的增殖能力和迁移能力强(P<0.001，P=0.002)。过表达TIP30质粒转染的cal-27-pTIP30细胞株TIP30 mRNA和蛋白的表达均明显高于cal-27-pc3.0细胞株 (P=0.001，P=0.003)，且细胞的增殖能力和迁移能力弱(P<0.001，P=0.005)。结论：OPN能促进cal-27细胞的增殖和迁移，TIP30抑制cal-27细胞的增殖和迁移。 |
| 英文摘要: |
| ABSTRACT Objective: To study the influence of up-regulated expressions of OPN (osteopontin) or TIP30 on the oral squamous cell carcinoma cal-27 cells. Methods: The TIP30 and OPN were overexpressed in the cal-27 cell lines respectively by infection or transfection technology. Real-time fluorescent quantitative PCR (RT-PCR) and western blot were used to detect the TIP30 and OPN mRNA and protein expressions. The proliferation ability was tested by CCK-8 tests, while the migration ability was tested by cell scratch experiment tests. Results: The expression of OPN mRNA and protein in cal-27-shOPN cell line was higher than those in the cal-27-shN cell lines(P<0.001, P=0.002). The proliferation and migration ability of cal-27-shOPN cell line were better than those of cal-27-shN cell line (P<0.001, P=0.002). The expression of TIP30 mRNA and protein in cal-27-pTIP30 cell line were higher than in the cal-27-pc3.0 cell line (P=0.001, P=0.003). The proliferation and migration ability of cal-27-pTIP30 cell line were worse than those of the cal-27-pc3.0 cell line (P<0.001, P=0.005). Conclusion: OPN could improve the ability of proliferation and migration of cal-27 cell line, while TIP30 could reduce the ability of proliferation and migration of cal-27 cell line. |
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