| 刘清君,田旭东,武正权,孙 平,李彦龙.三黄泻心汤对胃癌细胞增殖的影响[J].,2017,17(31):6028-6032 |
| 三黄泻心汤对胃癌细胞增殖的影响 |
| Effect of Sanhuang Xiexin Decoction on the Proliferation of Gastric Cancer Cells |
| 修订日期：2017-03-23 |
| DOI：10.13241/j.cnki.pmb.2017.31.007 |
| 中文关键词: 三黄泻心汤 胃癌细胞 幽门螺旋杆菌 增殖 lncRNA H19 |
| 英文关键词: Sanhuang Xiexin Decoction Gastric cancer cell Helicobacter pylori Proliferation LncRNA H19 |
| 基金项目:国家自然科学基金项目（81302966） |
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| 摘要点击次数: 250 |
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| 中文摘要: |
| 摘要 目的：探讨三黄泻心汤对胃癌细胞增殖的影响和机制。方法：以体外培养的胃癌细胞系HGC-27为研究对象，给予三黄泻心汤处理后，采用流式细胞仪检测三黄泻心汤对胃癌细胞系HGC-27细胞周期的影响，免疫荧光检测HGC-27细胞Ki67的表达，定量PCR实验检测增殖相关非编码RNA H19表达的影响。结果：当没有幽门螺旋杆菌时，三黄泻心汤对胃癌细胞系的细胞周期、Ki67及lncRNA H19的表达均无显著影响；而当存在一定浓度的幽门螺旋杆菌时，三黄泻心汤时处于分裂间期和前期(G0/G1期)的细胞的百分比显著的升高，处于DNA复制(S期)的细胞比例及Ki67、lncRNA H19的表达均明显的降低(P<0.05)。结论：在幽门螺旋杆菌存在时，三黄泻心汤显著抑制胃癌细胞系的增殖，可能与降低长链非编码RNA H19的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect and mechanism of Sanhuang Xiexin Decoction on the proliferation of gastric cancer cells. Methods: The gastric cancer cell line HGC-27 was cultured and treated with Sanhuang Xiexin Decoction. The effect of Sanhuang Xiexin Decoction on the cell cycle of HGC-27 was detected by flow cytometry. The expression of Ki67 in HGC-27 cells was detected by immunofluorescence, and the expression of proliferation-associated non-coding RNA (H19) was detected by qRT-PCR. Results: In the absence of Helicobacter pylori, Sanhuang Xiexin Decoction had no significant effect on the cell cycle, Ki67 expression and lncRNA H19 in gastric cancer cell lines. While there was a certain concentration of H. pylori, (G0/G1 phase), the percentage of cells in DNA replica- tion (S phase) and the expression of Ki67 and lncRNA H19 were significantly decreased (P<0.05). Conclusion: Sanhuang Xiexin Decoc- tion could significantly inhibit the proliferation of gastric cancer cell lines in the presence of Helicobacter pylori, which might be related to the decrease of H19 expression. |
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