段秋霞,栾 晓,郭菲菲,孙向荣,徐 珞.大鼠ARC-BMA nesfatin-1神经通路及对GD神经元放电活动和胃运动的影响[J].,2017,17(27):5227-5232 |
大鼠ARC-BMA nesfatin-1神经通路及对GD神经元放电活动和胃运动的影响 |
Nesfatin-1 Signaling in the Basom Edial Amygdala Modulates the Gastric Distension-sensitive Neurons Discharge and Decreases Gastric Motility by the Arcuate Nucleus |
投稿时间:2017-03-25 修订日期:2017-04-21 |
DOI:10.13241/j.cnki.pmb.2017.27.007 |
中文关键词: Nesfatin-1 杏仁核 弓状核 胃牵张敏感神经元 胃运动 |
英文关键词: Nesfatin-1 Amygdala Arcuate nucleus Gastric distension responsive neurons Gastric motility |
基金项目:国家自然科学基金项目(81470815,81270460,81500414);山东省优秀中青年科学基金项目(BS2014YY009);青岛市科技局项目(14-2-3-3-nsh) |
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中文摘要: |
摘要 目的:探讨弓状核(ARC)-杏仁核(BMA)间nesfatin-1神经通路的构成及其对胃牵张敏感(GD)神经放电活动和胃运动的影响。方法:逆行追踪结合免疫组化观察ARC-BMA间nesfatin-1神经通路;细胞外放电记录,观察nesfatin-1对GD神经元放电活动的影响及电刺激ARC对BMA内GD神经元放电活动的影响;在体胃运动研究,观察nesfatin-1对胃运动及胃排空的影响及电刺激ARC对胃运动的影响。结果:大鼠ARC-BMA间存在nesfatin-1神经通路;BMA微量注射Nesfatin-1能够促进GD-E神经元放电(4.25±1.02 Hz vs. 5.32±1.17 Hz,P<0.01),抑制GD-I神经元放电(3.73±0.92 Hz vs. 2.64±0.86 Hz,P<0.01),并且胃收缩频率及幅度下降,nesfatin-1的这些效应可被SHU9119部分阻断;电刺激ARC后,BMA内nesfatin-1反应性GD神经元放电频率增加(GD-E: 5.14±1.32 Hz vs. 6.75±1.84 Hz,P<0.05;GD-I:2.84±0.86 Hz vs. 4.05±1.12 Hz,P<0.05),并且胃收缩频率和幅度增强。结论:ARC-BMA间nesfafin-1通路可调控大鼠胃牵张敏感神经元放电活动和胃运动,该效应可能与黑色素信号通路有关。 |
英文摘要: |
ABSTRACT Objective: This study aimed to explore the effects of nesfatin-1 on gastric distension (GD)-sensitive neurons in the basomedial amygdala (BMA) and the potential mechanism for nesfatin-1 to regulate gastric motility through the arcuate nucleus (Arc). Methods: The projection of nerve fiber and expression of nesfatin-1 were observed by retrograde tracing and fluo-immunohistochemistry staining; The nuclei microinjection and nuclei electrical stimulation, extracellular discharges of single unit neuron were used to observe the effects of nesfatin-1 on the GD neurons; Gastric motility recording in vivo were used to monitor the effects of nesfatin-1 on the ampli- tude of constriction and frequency of gastric motility in conscious rats. Results: NUCB2/Nesfatin-1/fluorogold-double labeled neurons were from ARC to BMA; Nesfatin-1 could excited the firing rate of most of the GD-E neurons (4.25±1.02 Hz vs. 5.32±1.17 Hz, P<0.01) and decreased the firing rate of most of the GD-I neurons(3.73±0.92 Hz vs. 2.64±0.86 Hz, P<0.01), inhibited the gastric motility, amplitude and frequency, SHU9119 could weaken the responses induced by nesfaton-1; Electrical stimulation of the Arc, the firing rate of nesfatin-1-induced GD-response neurons (GD-E: 5.14±1.32 Hz vs. 6.75±1.84 Hz, P<0.05; GD-I: 2.84±0.86 Hz vs. 4.05±1.12 Hz, P<0.05) and the gastric amplitude and frequency were increase. Conclusion: It was suggested that nesfatin-1 in the BMA plays an important role in decreasing gastric motility and the Arc may be involved in this regulation process. |
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