| 石 嵩,栾 晓,柳 洋,郭菲菲,徐 珞.大鼠下丘脑LHA-PVN nesfatin-1神经通路构成及对胃运动的影响[J].,2017,17(25):4831-4836 |
| 大鼠下丘脑LHA-PVN nesfatin-1神经通路构成及对胃运动的影响 |
| The Effects of Nesfatin-1 in the Paraventricular Nucleus on Gastric Motility and Its Potential Regulation by the Lateral Hypothalamic Area in Rats |
| 投稿时间：2017-03-03 修订日期：2017-03-28 |
| DOI：10.13241/j.cnki.pmb.2017.25.008 |
| 中文关键词: 胃牵张敏感神经元 胃运动 下丘脑外侧核 nesfatin-1 下丘脑室旁核 |
| 英文关键词: Gastric distension responsive neurons Gastric motility Lateral hypothalamic area Nesfatin-1 Paraventricular nucleus |
| 基金项目:国家自然科学基金项目（81470815，81270460，81500414）；山东省优秀中青年科学基金项目（BS2014YY009）；青岛市科技局项目（14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要 目的：阐述LHA和PVN间nesfatin-1神经通路的构成，探讨PVN nesfatin-1对胃收缩幅度及频率的影响及潜在机制。方法：采用荧光金逆行追踪结合荧光免疫组化技术，观察LHA-PVN间nesfatin-1神经通路构成；采用细胞外放电记录，观察nesfatin-1对GD放点活动的影响；通过在体胃运动技术，观察nesfatin-1对清醒自由活动大鼠胃收缩幅度和频率的影响。结果：Nesfatin-1能够抑制GD-E神经元放电（1.97±0.12 Hz vs. 1.15±0.07 Hz）促进GD-I神经元放电（1.74±0.10 Hz vs. 3.04±0.18 Hz），H4928能够部分阻断nesfatin-1对GD神经元（GD-E：1.38±0.08 Hz，P<0.05 vs. nesfatin-1; GD-I：2.49±0.15 Hz，P<0.05 vs. nesfatin-1）的影响；PVN内微量注射nesfatin-1能够抑制大鼠胃运动，呈量效依赖关系；LHA和PVN间有nesfatin-1神经纤维联系；电刺激LHA后，GD神经元放电频率增加（GD-E：2.06±0.12 Hz vs. 4.23±0.21 Hz，GD-I：1.61±0.09 Hz vs. 4.83±0.25 Hz），预先向PVN注射抗NUCB2/nesfatin-1抗体后，GD-E神经元放电频率减弱（4.91±0.25 Hz vs. 4.23±0.21 Hz），而GD-I神经元放电频率增强（4.15±0.18 Hz vs. 4.83±0.25）。结论：PVN内nesfatin-1可调控大鼠GD神经元放电活动及胃运动，该效应受LHA调控。 |
| 英文摘要: |
| ABSTRACT Objective: The current study investigated the effects of nesfatin-1 in the hypothalamic paraventricular nucleus (PVN) on gastric motility and the regulation of the lateral hypothalamic area (LHA). Methods: The projection of nerve ?ber and expression of nesfatin-1 were observed by retrograde tracing and fuo-immunohistochemistry staining; The nuclei microinjection and nuclei electrical stimulation, extracellular discharges of single unit neuron were used to observe the effects of nesfatin-1 on the GD neurons; Gastric motil- ity recording in vivo were used to monitor the effects of nesfatin-1 on the amplitude of constriction and frequency of gastric motility in conscious rats. Results: Nesfatin-1 inhibited the majority of the GD-E neurons（1.97±0.12 Hz vs. 1.15±0.07 Hz） and excited GD-I neu- rons（1.74±0.10 Hz vs. 3.04±0.18 Hz） in the PVN, which were weakened by oxytocin receptor antagonist H4928（GD-E: 1.38±0.08 Hz, P<0.05 vs. nesfatin-1; GD-I: 2.49±0.15 Hz, P<0.05 vs. nesfatin-1）. Gastric motility experiments showed that administration of nes- fatin-1 in the PVN decreased gastric motility. Retrograde tracing and immunofuorescent staining showed that nucleobindin-2/nesfatin-1 and fuorogold double-labeled neurons were observed in the LHA. Electrical LHA stimulation excited the firing rate of GD-responsive neurons（GD-E: 2.06±0.12 Hz vs. 4.23±0.21 Hz, GD-I: 1.61±0.09 Hz vs. 4.83±0.25 Hz） in the PVN. Pre-administration of an anti- nucleobindin-2/nesfatin-1 antibody in the PVN strengthened gastric motility, decreased GD-E neurons（1.74±0.10 Hz vs. 3.04±0.18 Hz） and excited the discharging of the GD-I neurons（4.15±0.18 Hz vs. 4.83±0.25） induced by electrical stimulation of the LHA. Conclusion: Nesfatin-1 in the PVN could serve as an inhibitory factor to inhibit gastric motility, which might be regulated by the LHA. |
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