| 赵 易,赵庆丽,马 骥,蔡 黔,张 更.RNA结合蛋白QKI-5对乳腺癌细胞MCF-7增殖的影响研究[J].,2017,17(25):4816-4819 |
| RNA结合蛋白QKI-5对乳腺癌细胞MCF-7增殖的影响研究 |
| Effect of RNA Binding Protein of QKI-5 on Breast Cancer Cell MCF-7 of Proliferation |
| 投稿时间：2017-03-01 修订日期：2017-03-27 |
| DOI：10.13241/j.cnki.pmb.2017.25.004 |
| 中文关键词: QKI-5 乳腺癌 免疫印迹实验 MTT实验 |
| 英文关键词: QKI-5 Breast cancer Western blot MTT assay |
| 基金项目:国家自然科学基金面上项目（81101926） |
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| 中文摘要: |
| 摘要 目的：检测RNA 结合蛋白QKI-5在乳腺癌细胞中的表达水平以及对癌细胞增殖能力的抑制作用。方法：通过免疫印迹实验检测QKI-5在不同乳腺癌细胞株中的表达水平，通过慢病毒感染构建能够稳定过表达QKI-5基因的细胞株，使用MTT，流式细胞仪检测细胞周期来观察过表达QKI-5对细胞增殖能力及周期的影响。结果：MCF-7细胞在三株乳腺癌细胞中QKI-5表达水平相对最低，MTT实验结果显示与对照相比，过表达QKI-5的MCF-7细胞增殖能力出现显著降低P<0.05，同时细胞周期检测显示过表达QKI-5的MCF-7细胞组出现了明显的G1期阻滞，进入S期G2/M期细胞减少。结论：在乳腺癌中QKI-5的高表达可能通过抑制癌细胞周期致使细胞增殖变缓，从而导致肿瘤生长受限。 |
| 英文摘要: |
| ABSTRACT Objective: To check RNA binding protein QKI-5's expression level in breast cancer cells and inhibiting effect on can- cer cell proliferation. Methods: QKI-5's expression level was checked in different breast cancer cells by Western blotting, cancer cells of overexpressed QKI-5 gene could be stabilized by slow virus infection construction, MTT and FCM were used to check cell period to ex- press QKI-5's influence on cell proliferation and period. Results: MCF-7 cells have relatively low QKI-5 expression level in three breast cancer cells, MTT experiment result has obvious reduced influence on QKI-5's expression of MCF-7 cell proliferation P<0.05, mean- while, cell period inspection shows that overexpressed QKI-5's MCF-7 cells have obvious G1 retardant, cells in S and G2/M periods are reduced. Conclusion: High expression of QKI-5' in breast cancer could cause slow cell proliferation by inhibiting cancer cell period, hence causing limited tumor growth. |
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