| 薛峥峰,赵力芳,马 甜,李元霞,庞随军,谢坤霞.Galectin-7在哮喘儿童支气管黏膜中的表达及对支气管上皮细胞凋亡的影响[J].,2017,17(22):4239-4242 |
| Galectin-7在哮喘儿童支气管黏膜中的表达及对支气管上皮细胞凋亡的影响 |
| The Expression of Galectin-7 in Bronchial Mucosa of Asthmatic Children and Its Effect on the Apoptosis of Bronchial Epithelial Cells |
| 投稿时间：2017-01-09 修订日期：2017-01-30 |
| DOI：10.13241/j.cnki.pmb.2017.22.008 |
| 中文关键词: 哮喘 支气管黏膜 凋亡 Galectin-7 |
| 英文关键词: Asthma Bronchial mucosa Apoptosis Galectin-7 |
| 基金项目:陕西省自然科学基础研究计划项目(2014JM4141) |
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| 中文摘要: |
| 摘要 目的：探讨半乳糖凝集素-7(Galectin-7)在哮喘儿童支气管黏膜中的表达及对支气管上皮细胞凋亡的影响。方法：收集哮喘儿童支气管黏膜及支气管扩张非哮喘儿童支气管黏膜，Western blot检测其Galectin-7的表达。体外培养人支气管上皮细胞，分为正常组、对照组、感染组和实验组，正常组用正常的人支气管上皮细胞，对照组细胞用转染siRNA control后的人支气管上皮细胞，感染组细胞用RSV感染后的人支气管上皮细胞，实验组细胞为RSV感染后并转染siRNA Galectin-7的人支气管上皮细胞。培养24 h后，检测各组细胞中Galectin-7蛋白表达，并采用流式细胞术检测各组细胞的凋亡情况，Western blot检测细胞中Bcl-2、Bax、STAT3、p-STAT3蛋白的表达。结果：哮喘儿童支气管黏膜中Galectin-7的表达明显高于非哮喘儿童支气管黏膜组织(P<0.01)。正常组和对照组Galectin-7水平比较差异无统计学意义(P>0.05)，感染组Galectin-7、Bax表达和细胞凋亡率均明显高于正常组，而Bcl-2、p-STAT3的表达均明显低于正常组(P<0.01)，实验组Galectin-7、Bax表达和细胞凋亡率明显低于感染组，而Bcl-2、p-STAT3的表达均明显高于感染组(P<0.01)。结论：Galectin-7在哮喘儿童支气管黏膜中表达上调，可能通过活化STAT3，促进支气管上皮细胞凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of Galectin-7 in the bronchial mucosa of asthmatic children and its effect on the apoptosis of bronchial epithelial cells. Methods: Bronchial mucosa of asthmatic children and children with bronchial dilation were collected and the expression of Galectin-7 was detected by Western blot. Human bronchial epithelial cells were cultured in vitro, the cells were transfected with Galectin-7 siRNA to interfere the Galectin-7 expression, while siRNA control was transfected as the control group. The experiment was divided into normal group, control group, infected group and experimental group. The normal group was normal human bronchial epithelial cells, the cells in the control group was transfected with siRNA control, the infected group was infected with RSV, the experimental group was transfected with Galectin-7 siRNA and infected with RSV. After 24h culture, Galectin-7 protein expression and cell apoptosis were detected in the cells of each group . Western blot was used to detected the expression of Bcl-2, Bax, STAT3 and p-STAT3. Results: The expression of Galectin-7 in bronchial mucosa of asthmatic children was significantly higher than that of the non asthmatic children (P<0.01). There was no significant difference in the Galectin-7 level between the normal group and the control group (P>0.05). The levels of Galectin-7, Bax and apoptosis in the infected group were significantly higher than those in the normal group, while the levels of p-STAT3 and Bcl-2 were significantly lower than those in the normal group (P<0.01). The levels of Galectin-7, Bax and apoptosis in the experimental group were significantly lower than those in the infected group, while the levels of p-STAT3 and Bcl-2 were significantly higher than those in the infected group (P<0.01). Conclusion: The expression of Galectin-7 was up-regulated in the bronchial mucosa of asthmatic children, which might promote the apoptosis of bronchial epithelial cells by activating STAT3. |
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