| 蔡承魁,田丽颖,韩 康,贠 喆,马保安.miR-15a-5p和miR-16-5p在骨肉瘤组织中的表达分析[J].,2017,17(20):3827-3830 |
| miR-15a-5p和miR-16-5p在骨肉瘤组织中的表达分析 |
| Expression of miR-15a-5p and miR-16-5p in Osteosarcoma |
| 投稿时间：2017-02-19 修订日期：2017-03-13 |
| DOI：10.13241/j.cnki.pmb.2017.20.006 |
| 中文关键词: miR-15a-5p miR-16-5p 骨肉瘤 |
| 英文关键词: miR-15a-5p miR-16-5p Osteosarcoma |
| 基金项目:国家自然科学基金项目（81072194） |
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| 摘要点击次数: 388 |
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| 中文摘要: |
| 摘要 目的：使用microRNAs基因芯片及实时定量PCR法测定骨肉瘤组织中miR-15a-5p和miR-16-5p的相对表达含量，并与瘤旁组织对比，分析骨肉瘤细胞内miR-15a-5p和miR-16-5p的表达变化。方法：选取34例骨肉瘤组织蜡块样本，使用microRNAs基因芯片观察miR-15a-5p和miR-16-5p在骨肉瘤和瘤旁组织内的表达差异；实时定量PCR法测定骨肉瘤组织和瘤旁组织中miR-15a-5p和miR-16-5p的相对表达含量，并将两种结果对比分析。结果：microRNAs基因芯片结果显示，在骨肉瘤组织中，miR-15a-5p在肿瘤中的表达较瘤旁组织低1.79倍，miR-16-5p较瘤旁组织低1.62倍。实时定量PCR实验结果表明， miR-15a-5p和miR-16-5p表达较瘤旁组织降低，差异有统计学意义（P<0.05）。经过统计学计算，miR-15a-5p在肿瘤中的表达较瘤旁组织低3.14倍，miR-16-5p较瘤旁组织低5.65倍。结论：在骨肉瘤中，miR-15a-5p和miR-16-5p表达含量降低，提示这两种microRNAs在骨肉瘤中可能做为抑癌因子存在。 |
| 英文摘要: |
| ABSTRACT Objective: Use microRNA microarray chip and real time quantitative PCR method to analysis the relative expression levels of miR-15a-5p and miR-16-5p, then compare the difference between osteosarcoma paraffin block samples and peritumoral tissue. Methods: 34 cases of osteosarcoma paraffin block samples were chosen, microRNAs microarray chip and real time quantitative PCR method was used to measure the relative expression levels of miR-15a-5p and miR-16-5p. Results: microRNAs microarray chip results showed that the expression level of miR-15a-5p (miR-16-5p) in tumor tissue was 1.79 (1.62) times lower than the adjacent tissue. Real time quantitative PCR results showed that compare with tumor adjacent tissues, the expression level of miR-15a-5p and miR-16-5p was low in osteosarcoma paraffin block samples. The results were considered significant through statistical analysis (P<0.05). Further analysis results showed that the expression level of miR-15a-5p (miR-16-5p) in tumor tissue was 3.14 (5.65) times lower than the adjacent tissue. Conclusion: miR-15a-5p and miR-16-5p was low expressed in osteosarcoma cells, which suggest that these two microRNAs could be act as oncogene. |
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