| 温伟伟,李洪波,姜英健,秦凯强,梁忆波,马 雷,郑红梅,张佃良.Sprouty-2对胃癌细胞上皮间质转化及侵袭转移的影响[J].,2017,17(17):3250-3254 |
| Sprouty-2对胃癌细胞上皮间质转化及侵袭转移的影响 |
| Effect of Sprouty-2 on the Epithelial-mesenchymal Transition, Migration and Invasion of Human Gastric Cancer |
| 投稿时间：2017-01-08 修订日期：2017-02-02 |
| DOI：10.13241/j.cnki.pmb.2017.17.012 |
| 中文关键词: 胃癌 Sprouty-2 上皮-间质转化 侵袭 转移 |
| 英文关键词: Stomach Neoplasms Sprouty-2 Epithelial-mesenchymal transition Invasion Migration |
| 基金项目:国家自然科学基金项目（81270448，81470890） |
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| 中文摘要: |
| 摘要 目的：研究Sprouty2(SPRY2)基因在胃癌肿瘤细胞上皮间质转化(EMT)和侵袭转移的影响。方法：体外培养人胃癌细胞(BGC-823)，采用慢病毒介导的shRNA沉默SPRY2基因，并用实时定量PCR与Western blot检测其SPRY2、E-钙黏蛋白(E-cadherin)、波形蛋白(vimentin)的表达，采用细胞划痕实验、Transwell实验检测SPRY2基因沉默后的胃癌细胞侵袭转移能力变化。结果：在慢病毒介导shRNA沉默SPRY2基因的人胃癌BGC-823细胞中，SPRY2的mRNA和蛋白表达明显降低(P＜0.05)，SPRY2沉默后人胃癌细胞E-cadherin的蛋白表达增多(P＜0.05)，vimentin的蛋白表达减少(P＜0.05)。此外，SPRY2沉默后，胃癌细胞迁移能力和侵袭能力明显减弱(P值均P＜0.05)。结论：Sprouty-2基因通过调节E-cadherin与vimentin的表达参与胃癌细胞的上皮-间质转化，进而促进胃癌细胞的迁移与侵袭。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of suppressed Sprouty2 (SPRY2) on the epithelial-mesenchymal transition (EMT), migration and invasion of human gastric cancer. Methods: Human gastric cancer line (BGC-823) was transfected with shRNA-SPRY2 mediated by recombinant lentivirus to establish a cell line (shRNA- SPRY2- BGC-823) which was stably suppressed to express SPRY2. The expression level of SPRY2, EMT-related marker proteins (E-cadherin and vimentin) were examined using real-time PCR and West- ern blot in human BGC-823 cells. In addition, migration and invasion assays were performed to assess the effect of SPRY2 on the BGC-823 cells using Matrigel-coated plate, transwell membrane chamber, and wound healing models, respectively. Results: The human gastric cancer line (shRNA- SPRY2- BGC-823) was successfully constructed by lentivirus stable transfection to suppress the expression levels of SPRY2. The expression of SPRY2 was significantly decreased after knockdown of SPRY2 in BGC-823 cells compared with control-shRNA and non-transfection. The results from real-time PCR and Western blot indicated that the mRNA and protein expression of the epithelial cell marker E-cadherin was significantly increased, and the mesenchymal cell markers vimentin was significantly de- creased after knockdown of SPRY2 in BGC-823 cells. Moreover, after SPRY2 knockdown, abilities of migration and invasion were sig- nificantly attenuated in shRNA- SPRY2- BGC-823 cells. Conclusion: SPRY2 knockdown significantly promote the EMT by regulating the expressions of E-cadherin and vimentin, which further enhanced the migration and invasion of human gastric cancer in vitro. |
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