| 杨 克,李 龙,乔中原,樊 荣,杨 林.Orexin-A对大鼠脑缺血再灌注损伤的保护作用[J].,2017,17(17):3246-3249 |
| Orexin-A对大鼠脑缺血再灌注损伤的保护作用 |
| Protective Effect of Orexin-A on the Cerebral Ischemia Reperfusion Injury in Rats |
| 投稿时间：2017-01-05 修订日期：2017-02-07 |
| DOI：10.13241/j.cnki.pmb.2017.17.011 |
| 中文关键词: Orexin-A 脑缺血再灌注损伤 神经保护 |
| 英文关键词: Orexin-A Cerebral ischemia reperfusion injury Neuroprotection |
| 基金项目:国家自然科学基金青年基金项目(81401138) |
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| 中文摘要: |
| 摘要 目的：研究orexin-A对缺血再灌注大鼠脑损伤的保护作用。方法：取成年雄性大鼠6只，观察MCAO前和MCAO后2 h、24 h的生理学参数，界定后续指标参考时间。另取20只大鼠随机分为MCAO组、vehicle组、orexin-A 50 μg/kg组和orexin-A 100 μg/kg组(n=5)，于缺血再灌注24 h后评估大鼠神经功能学评分和脑梗死容积。再取60只大鼠同样分成4组，(各组n=15)，每组在术前、手术后6 h、24 h(各时间点n=5)取脑组织匀浆离心，检测上清液中谷胱甘肽过氧化物酶(GSH-PX)和丙二醛(MDA)的含量。结果：①大鼠MCAO术前、术后2 h、24 h生理参数比较无统计学意义(P>0.05)，提示脑保护参考指标在MCAO后24 h内不受影响。②与MCAO组、vehicle组相比，orexin-A 50和100 μg/kg降低神经功能评分(P<0.05)且梗死容积缩小(P<0.05)；术前、术后6 h和术后24 h，脑匀浆中GSH-PX活性升高，MDA含量降低(P<0.05)。结论：Orexin-A可能通过降低脑内自由基水平，控制脂质过氧化物酶从而对脑缺血再灌注损伤起保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To evaluate the protective effect of orexin-A on the cerebral ischemia reperfusion injury in rats. Methods: The physiological parameters were observed to define time rang by six rats before MCAO and after MCAO 2 h, 24 h. Twenty rats were randomly divided into middle cerebral artery occlusion (MCAO) group, vehicle group, orexin-A 50 μg/kg and orexin-A 100 μg/kg group (n=5). Neurological dysfunction scores (NDS) and infarct volume were measured at 24h after ischemia-reperfusion. The other sixty rats were also divided into 4 groups (n=15 each group), the level of glutathione peroxidase (GSH-PX) and maleic diadehyde (MDA) in brain plasm were detected pre-operation and at 6 h, 24 h after ischemia-reperfusion (n=5 each time point). Results: ①No significant differentce was found in the pyhsiological parameters of rats before MCAO and at 2 h, 24 h after MCAO (P>0.05), suggesting that the follow indexes of cerebral protection hadn't been influenced within 24 h after MCAO. ②Compared with the MCAO group and vehicle group, the NDS (P<0.05) and the percentage of brain infarct were better in the orexin-A 50 and 100 μg/kg group(P<0.05); the concentration of GSH-PX was increased and MDA was decreased in orexin-A 50 and 100 μg/kg group (P<0.05). Conclusion: Orexin-A might play a protective role in the cerebral ischemia reperfusion injury in rats by increasing the concentration of antiperoxidase and decreasing the level of oxygen free radicals. |
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