| 张海佳,沙素梅,徐 斌,王 虎,许 冰,王国栋,蒋铭佐,聂勇战,吴开春,李孟彬.IL-17在黏附侵袭性大肠杆菌感染小鼠结肠过程中的作用机制研究[J].,2017,17(17):3211-3215 |
| IL-17在黏附侵袭性大肠杆菌感染小鼠结肠过程中的作用机制研究 |
| The Role and Mechanism of IL-17 in the Colon Infected by Adherent Invasive Escherichia coli LF82 |
| 投稿时间：2017-01-04 修订日期：2017-01-26 |
| DOI：10.13241/j.cnki.pmb.2017.17.003 |
| 中文关键词: 炎症性肠病 大肠杆菌LF82 白细胞介素17 |
| 英文关键词: Inflammatory bowel disease Escherichia coli LF82 IL-17 |
| 基金项目:国家自然科学基金项目（81500423，81370504，81502082，81600443） |
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| 中文摘要: |
| 摘要 目的：评价IL-17在黏附侵袭性大肠杆菌感染小鼠结肠过程中的作用及其可能机制。方法：选择野生型及IL-17基因敲除的SPF级C57BL/6小鼠并随机分成4组，分别给予不同的处理：(1)野生小鼠+单纯蒸馏水灌胃处理组；(2)野生小鼠+E.coli LF82(1×109/CFU/只)灌胃10天处理组；(3)IL-17敲除小鼠+单纯蒸馏水灌胃处理组；(4)IL-17敲除小鼠+E.coli LF82灌胃10天处理组。从以下5个方面评价各组小鼠的炎症反应和IL-17水平：(1)组织病理评分评估炎症反应严重程度；(2)透射电镜下观察结肠上皮细胞的超微结构；(3)免疫组织化学检测结肠分泌的IL-17；(4)PCR检测小鼠结肠中IL-17mRNA表达；(5)ELISA检测结肠组织中IL-17的含量。结果：定植了E.coli LF82的IL-17敲除小鼠肠道炎症程度和超微结构损伤较野生型小鼠更加严重(P＜0.05)。与未经E.coli LF82处理组相比，定植了E.coli LF82的野生小鼠肠道中IL-17mRNA和IL-17含量明显升高(P＜0.05)。结论：IL-17在E.coli LF82在黏附侵袭结肠粘膜过程中的保护作用，IL-17是针对AIEC菌株E.coli LF82免疫的重要效应物，而结肠局部分泌增加的IL-17会改善感染的结果。 |
| 英文摘要: |
| ABSTRACT Objective: To assess the role of IL-17 in the colon of mice infected by adherent-invasive Escherichia coli (AIEC) strain E.coli LF82. Methods: The wild-type (WT) mice and IL-17KO (IL-17 knockout) mice placed in specific pathogen free (SPF) housing were randomly divided into 4 groups: (1) WT mice + distilled water; (2) WT mice + E. coli LF82 by oral gavage for 10 days; (3) IL-17KO mice + distilled water; (4) IL-17KO mice + E.coli LF82 by oral gavage for 10 days. Four aspects of the treatment response were evaluated in each group: (1) histological score; (2) transmission electron microscope (TEM) to explore the ultrastructure; (3) the secretion of IL-17 detected by immune histochemistry; (4) the relative quantitative analysis of IL-17 in the colon detected by RT-PCR; (5) the quantitative analysis of IL-17F in the colon detected by ELISA. Results: Compared with non-treatment group, the expression of IL-17 mRNA and the activity of IL-17 were significantly higher than that in WT mice (P<0.05). After E. coli LF82 treatment, compared with WT mice, the histological score and the ultrastructure in IL-17KO mice improved significantly (P<0.05). Conclusion: IL-17 protects colonic epithelial integrity in the colon infected by adherent invasive Escherichia coli LF82. IL-17 is an important effector of immunity to AIEC strain E.coli LF82, suggestting that an increased local production of IL-17 would improve the outcome of infection. |
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