何 宏,顾 婷,薛姗姗,王化宁,彭正午,周翠红.异氟烷对小鼠星型胶质细胞Sirt1和MAO-A基因表达的影响[J].,2017,17(15):2817-2820 |
异氟烷对小鼠星型胶质细胞Sirt1和MAO-A基因表达的影响 |
Effect of Isoflurane on the Expression of Sirt1/MAO-A in Cultured Astrocytes |
投稿时间:2016-12-11 修订日期:2016-12-29 |
DOI:10.13241/j.cnki.pmb.2017.15.005 |
中文关键词: 异氟烷 星形胶质细胞 Sirt1 MAO-A |
英文关键词: Isoflurane Astrocytes Sirt1 MAO-A |
基金项目:国家自然科学基金项目(81401109,81571309) |
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中文摘要: |
摘要 目的:探讨异氟烷对小鼠星形胶质细胞Sirt1和MAO-A基因表达的影响。方法:给予体外培养的新生小鼠原代星形胶质细胞不同浓度异氟烷处理,实验分为对照组和异氟烷处理组(0.5ISO、1.0ISO、1.5ISO),其中异氟烷组细胞分别给予0.5 MAC、1.0 MAC和1.5 MAC三个浓度的异氟烷处理2小时,对照组给予O2处理2小时,然后提取细胞RNA和蛋白检测Sirt1和MAO-A的mRNA和蛋白表达变化。结果:与对照组相比,异氟烷下调小鼠星形胶质细胞的Sirt1和MAO-A mRNA和蛋白水平,异氟烷浓度越大下调越明显。结论:异氟烷可抑制小鼠星形胶质细胞的Sirt1和MAO-A基因表达。 |
英文摘要: |
ABSTRACT Objective: To explore the effect of isoflurane on the expression of Sirt1/MAO-A of Astrocytes. Methods: Cultured as- trocytes from newborn mice were divided into control and isoflurane groups: control group was treated with O2 for 2 hours and isoflurane treatment groups (0.5ISO, 1.0ISO, 1.5ISO) were treated with 0.5 MAC, 1.0 MAC and 1.5 MAC of isoflurane for 2 hours respectively. Then total RNA and protein was extracted from cells and the mRNA and protein levels of Sirt1 and MAO-A were measured by Real-time PCR and Western blot respectively. Results: (1) Compared with the control, Isoflurane significantly down-regulated the mRNA as well as protein levels of Sirt1 and MAO-A in the cultured astrocytes; (2) The effect of isoflurane revealed a dose effect---compared with 0.5ISO group, cells treated with 1.0MAC and 1.5 MAC of isoflurane suffered more influence. Conclusion: Isoflurane exposure suppressed the expression of Sirt1/MAO-A of astrocytes from newborn mice brain, which is important for the isoflurane narcotherapy in depressive. |
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