| 段佳林,奚苗苗,牟 菲,蔺 瑞,赵美娜,卫 国,文爱东,张恩户.紫铆花素通过调节AMPK/GSK-3β信号通路抑制心肌缺血再灌注损伤[J].,2017,17(14):2616-2621 |
| 紫铆花素通过调节AMPK/GSK-3β信号通路抑制心肌缺血再灌注损伤 |
| Protective Effects and Possible Mechanism of Butein on Myocardial Ischemia Reperfusion Injury |
| 投稿时间：2016-12-06 修订日期：2017-01-03 |
| DOI：10.13241/j.cnki.pmb.2017.14.004 |
| 中文关键词: 紫铆花素 心肌缺血再灌注 AMPK GSK-3β |
| 英文关键词: Butein Myocardial Ischemia/reperfusion Injury AMPK GSK-3β |
| 基金项目:国家自然科学基金面上项目（81470174）；西京医院学科助推项目（XJZT15M19） |
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| 中文摘要: |
| 摘要 目的：研究紫铆花素对心肌缺血再灌注损伤的保护作用及其机制。方法：体外建立H9c2心肌细胞缺血再灌注模型，分为正常组、模型组、紫铆花素低、中和高剂量组（10，20 和40μM）。检测细胞存活率，LDH释放水平，试剂盒检测MDA、SOD、IL-1和IL-6水平，蛋白印迹法检测Bax，Bcl-2蛋白的表达以及AMPK和GSK-3β磷酸化水平。结果：与模型组比较，紫铆花素能够提高细胞存活率，减少LDH水平，降低MDA、IL-1和IL-6，增加SOD水平。减少Bax，Caspase-3蛋白的表达，增加 Bcl-2 蛋白的表达，提高 Bcl-2/Bax的比值(P <0.05)。同时，紫铆花素能够剂量依赖性的促进AMPK和GSK-3β磷酸化。进一步研究发现，紫铆花素的保护作用以及对GSK-3β的促磷酸化被AMPK抑制剂Compound C抵消。结论：紫铆花素能减轻心肌缺血再灌注损伤，抑制心肌细胞凋亡，其作用机制可能通过激活AMPK/GSK-3β信号通路，减轻氧化应激水平有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the protective effects and possible mechanism of butein on myocardial ischemia/reperfusion injury. Methods: H9c2 cells were divided into normal control group, model group, low-dose, medium-dose and high-dose butein groups (10, 20 and 40 μM). After the drug was administered to cells for 24h, the myocardial ischemia-reperfusion injury was established. Cell viability, LDH, MDA, SOD, IL-1 and IL-6 were tested. The protein expressions of AMPK, Bax, Bcl-2, and GSK-3β were assayed by western blotting. Results: Comparedwith model group, butein could improve the cell survival rate, reduce the release of LDH, decrease the levels of MDA, IL-1 and IL-6, increase the level of SOD, decrease the expression of Bax, Caspase-3, increase the expression of Bcl-2and the ratio of Bcl-2 /Bax (P <0.05). In further study, we found that these protective effects were abolished by Compound C. Conclusion: Butein has the protective effects on myocardial ischemia/reperfusion injury in vitro, which can inhibit the myocardial injury through activating AMPK/ GSK-3β pathway. |
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