| 孟 云,于建勃,庄德舒,毕良佳,张喆焱.透明质酸联合盐酸米诺环素对大鼠金黄色葡萄球菌感染创口的治疗效果[J].,2017,17(11):2024-2027 |
| 透明质酸联合盐酸米诺环素对大鼠金黄色葡萄球菌感染创口的治疗效果 |
| Effects of Hyaluronic Acid combined with Minocycline Hydrochloride on the Wound Infected by Staphylococcus Aureus in Rats |
| 投稿时间：2016-10-20 修订日期：2016-11-16 |
| DOI：10.13241/j.cnki.pmb.2017.11.006 |
| 中文关键词: 口腔颌面部软组织损伤 透明质酸联合盐酸米诺环素凝胶 创口愈合 肿瘤坏死因子α |
| 英文关键词: Oral and maxillofacial soft tissue injury Hyaluronic acid combined with minocycline hydrochloride gel Wound heal- ing TNF-α |
| 基金项目:国家自然科学基金项目（81670994） |
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| 中文摘要: |
| 摘要 目的：探讨透明质酸联合盐酸米诺环素对大鼠金黄色葡萄球菌感染创口的治疗效果。方法：选择32只Wistar大鼠，在背部脊柱两侧各制备1个全层皮肤缺损模型。创面接种细菌形成感染创口并将大鼠随机地分为4个组，每个组共有16个创口。实验组(1.5 %透明质酸联合0.1 %盐酸米诺环素凝胶治疗组)、对照组1(0.9 %生理盐水治疗组)、对照组2(1.5 %透明质酸凝胶治疗组)、对照组3(0.1 %盐酸米诺环素凝胶治疗组)。观察分析创口愈合情况及创口愈合率，HE染色组织形态学分析，免疫组织化学染色法分析创口中肿瘤坏死因子α的表达。结果：透明质酸联合盐酸米诺环素组创口愈合率为(78.13±3.04) %，显著高于生理盐水对照组、透明质酸对照组和盐酸米诺环素对照组(均P<0.05)；透明质酸联合盐酸米诺环素组创口可见大量成熟的纤维组织，生理盐水组创口可以观察到组织发生坏死，结构不清；透明质酸组可见富含血管的新生肉芽组织；盐酸米诺环素组可见大量纤维组织；透明质酸联合盐酸米诺环素组创口TNF-α表达为10.84±1.49，显著低于生理盐水对照组、透明质酸对照组和盐酸米诺环素对照组(均P<0.05)。结论：1.5 %透明质酸联合0.1 %盐酸米诺环素凝胶对大鼠金黄色葡萄球菌感染创口有较好的治疗效果。 |
| 英文摘要: |
| ABSTRACT Objective: This study explores the therapeutic effects that hyaluronic acid combined with minocycline hydrochloride in rats wound infected with Staphylococcus aureus. Methods: The full-thickness dermal excision models on two sides of spine were estab- lished on 32 wistar rats, wounds were inoculated with Staphylococcus aureus to form infected wound. There were 4 groups of rat and 16 in- fected wounds in each group, divided randomly. The experimental group(1.5 % hyaluronic acid combined with 0.1 % minocycline hy- drochloride gel treatment group, n=8), the first control group(0.9 % normal saline treatment group, n=8), the second control group (1.5 % hyaluronic acid gel treatment group, n=8) and the third control group(0.1 % minocycline hydrochloride gel treatment group, n=8). The wound healing condition and the rate of that related to rats were watched and detected, through the method of HE staining to fulfill histo- logical analysis and imunohistochemical staining of TNF-α were accomplished. Results: The rate of wound healing in the hyaluronic acid combined with minocycline hydrochloride group was (78.13±3.04) %, significantly higher related to that of normal saline group(P<0.05), hyaluronic acid group(P<0.05) and minocycline hydrochloride group(P<0.05). There existed a great quantity of mature fibrous tissue in the hyaluronic acid combined with minocycline hydrochloride group; the tissue necrosis and unclear structure were observed in the nor- mal saline group; in the hyaluronic acid group, the granulation tissue was rich in blood vessels; there existed a mass of fibrous tissue in the minocycline hydrochloride group. The expression of TNF-α in the hyaluronic acid combined with minocycline hydrochloride group was 10.84±1.49 significantly lower related to that of normal saline group(P<0.05), hyaluronic acid group(P<0.05) and minocycline hy- drochloride group(P<0.05). Conclusion: Our study show that 1.5 % hyaluronic acid combined with 0.1 % minocycline hydrochloride gel can have a promising therapeutic effect on the rats wound infected with staphylococcus aureus. |
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