| 余群 徐仁华 王鹤霖 郭建影 邓海腾.敲低HSP60 对结肠癌SW480 细胞增殖能力的影响及其机制探究[J].,2017,17(7):1201-1205 |
| 敲低HSP60 对结肠癌SW480 细胞增殖能力的影响及其机制探究 |
| The Effects of HSP60 Knockdown on the Proliferation of Colon CancerSW480 and its Mechanism |
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| DOI： |
| 中文关键词: 热休克蛋白60 结肠癌细胞 细胞增殖 细胞周期 |
| 英文关键词: HSP60 Colon cancer Cell proliferation Cell cycle |
| 基金项目:国家自然科学基金项目(NSFC 31270871)；中华人民共和国科技部(2014CBA02005&2014AA020907) |
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| 中文摘要: |
| 目的：研究热休克蛋白60 (HSP60)敲低对结肠癌SW480 细胞增殖的影响，并进一步探究其作用机制。方法：通过含HSP60
shRNA 载体的慢病毒感染加上流式细胞仪无菌分选的方法构建结肠SW480 HSP60 基因稳定RNA 干扰（RNAi）单克隆细胞系，
利用Western blot 和q-PCR验证结肠癌细胞中HSP60的敲低效率；使用CCK-8试剂检测结肠癌细胞增殖能力，并用流式细胞仪
检测其HSP60 敲低对细胞周期的影响。结果：Western blot 和q-PCR结果验证了HSP60 在结肠癌细胞中的敲低效率，与对照组细
胞相比，实验组细胞HSP60 的mRNA 水平和蛋白水平均降低了60%以上。CCK-8 实验结果表明，敲低HSP60 后SW480 细胞的
增殖能力下降了约70%；流式细胞周期实验显示敲低HSP60 后SW480 细胞中G0/G1 期、S 期、G2/M期的分布比例变化不大。结
论：敲低HSP60能够显著抑制SW480 细胞的增殖能力，而SW480细胞周期并没有发生明显变化，推测HSP60 的敲低引起的线
粒体损伤导致细胞生长速度变慢。 |
| 英文摘要: |
| Objective:To investigate the effects of HSP60 knockdown on the proliferation of colon cancer SW480 cells.Methods:Lentivirus containing the HSP60-directed shRNAs was used to construct a stable monoclonal HSP60 knockdown cell line in SW480. The
HSP60 knockdown efficiency was determined by western blotting and the theCCK-8 assay was used to analyze cell proliferation rates,
while flow cytometry was used to determine the cell cycle in HSP60-knocked down cells.Results:We established a stable cell line in
which HSP60 expression was silenced in SW480 cells as confirmed by western blotting and q-PCR. CCK-8 assay showed that HSP60
knockdown significantly inhibited the cell proliferation, whereas it had little effect on the cell cycle progression.Conclusion:HSP60
knockdown inhibits the cell proliferation in SW480 cells. so we speculate that the reduce of SW480 growing is due to mitochondrial
damage. |
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