| 熊湾湾 王纪莲 王绪宁.HMGA2 通过激活Wnt信号通路促进结直肠癌细胞增殖[J].,2017,17(3):414-417 |
| HMGA2 通过激活Wnt信号通路促进结直肠癌细胞增殖 |
| HMGA2 Promotes Colorectal Cancer Cell Proliferation through ActivatingWnt Signaling |
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| DOI： |
| 中文关键词: Wnt信号通路 HMGA2基因 结直肠癌 细胞增殖 |
| 英文关键词: Wnt signaling HMGA2 Colorectal cancer Cell proliferation |
| 基金项目: |
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| 中文摘要: |
| 目的：HMGA2(high-mobility group AT-hook 2)一个染色质蛋白，被报道在多种癌症中都发挥重要作用。本文研究染色体蛋白
HMGA2 对Wnt 信号传递的影响，及对结直肠癌细胞增殖的影响。方法：本文通过qRT-PCR 和免疫印迹法检测HMGA2 在结直
肠癌样本中mRNA和蛋白水平。用荧光素酶报告基因系统研究HMGA2 对Wnt信号通路的作用。用细胞增殖实验检测HMGA2
对结直肠癌细胞增殖的作用。结果：在我们检测的大部分结直肠癌样本里，HMGA2 表达水平升高；HMGA2 蛋白可上调Wnt 信号
通路荧光素酶报告基因TOPflash-luciferase 的表达，并呈现剂量依赖的形式。降低HMGA2 表达可抑制由Wnt3a、Dvl
（Dishevelled）、LiCl 以及茁catenin（S37A）引起的TOPflash-luciferase 报告基因表达上调作用。此外，SW480 中过量表达HMGA2
可以促进细胞增殖。结论：HMGA2 在结直肠癌中表达升高，HMGA2 在结直肠癌中通过增强Wnt 信号来促进结直肠癌细胞的增
殖。 |
| 英文摘要: |
| Objective:HMGA2 (high-mobility group AT-hook 2) is a chromosome protein which has been well-documented to
regulate cancer progression. We investigated whether and how HMGA2 affects Wnt signaling transduction process and the proliferation
of colorectal cancer cells.Methods:RT-qPCR and western blot were utilized to examine mRNA and protein expression levels changes of
HMGA2 in colorectal cancer tissues compared to nearby normal tissues. TOPflash reporter assay was conducted to detect the regulatory
function of HMGA2 in Wnt signaling. Cell proliferation was quantified by Cell Counting Kit-8 in SW480 cells infected by adenovirus
containing HMGA2 expression construct.Results:We found that both the mRNA and protein of HMGA2 were highly expressed in most
of the examined human colorectal cancer samples. HMGA2 increased the expression of TOPflash a dose-dependent manner. And knockdown
HMGA2 expression reduced Wnt3a-, LiCl-, 茁catenin- or Dvl (Dishevelled)-activated TOPflash-luciferase expression in colorectal
cancer cell line HCT-116. Furthermore, ectopic expression of HMGA2 promoted proliferation of colorectal cancer cell line SW480.Conclusion:HMGA2 gene was highly expressed in the majority of the examined colorectal cancer samples. HMGA2 can promote the proliferation
of colorectal cancer cells by up-regulating Wnt signaling. These results reveal the molecular and pathological functions of HMGA2
in colorectal cancer development and a potentially new regulatory effect on Wnt signaling. |
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