| Objective:To study influence of Ursolic Acid (UA) on bone formation and bone mineral in alcohol-induced
osteoporosis in rats.Methods:Adult male wistar rats (n=10 for each group) were randomly divided into blank control group, UA control
group, model group, UA low-, medium-, high group and were respectively given saline, 150 mg/kg UA, 50%alcohol, 50 mg/kg UA, 100
mg/kg UA, 150 mg/kg UA. UA control group was administered an equal volume of saline as the blank control group, and UA low-,
medium-, high group were received an equal volume of alcohol as the alcohol model group. The rats were administered for 8 weeks. The
content of serumphosphorus (P) was detected by phosphate method. The content of serum calcium (Ca) was detected by chromatometry
method. Serumbone gla protein (BGP) and bone morphogenetic protein 2 (BMP-2) were detected by ELISA.Results:Compared with the
blank control group, the contents of serumCa, P, BGP and BMP-2 were reduced in the model group (P<0.05). Compared with the model
group, the contents of serum Ca, P, BGP and BMP-2 were significantly increased in the medium-, and high-dose group (P<0.05). The
result of HE staining revealed that bone trabecular was rules, density and uniform thickness in blank control group, and bone trabecular
was irregular, sparse, and fracture in the model group. Compared with the model group, bone trabecular was intact, arranged regularly
and continuous in the medium-, and high-dose group.Conclusion:Alcohol plays its role in AOP rats by reducing bone formation and
promoting bone mineral loss. UA has antiosteoporotic effects on AOP rats by promoting bone formation and reversing bone mineral loss. |
