| 李建民 杨柳 王业秋 张宁△ 陈丽娟△ 胡中花 徐艳明 李娜 熊辉.补骨脂素对中波紫外线导致人皮肤HaCaT细胞光老化的保护作用[J].,2017,17(2):214-219 |
| 补骨脂素对中波紫外线导致人皮肤HaCaT细胞光老化的保护作用 |
| Protective Role of Psoralen Grain of on Light Aging of Human Skin HaCaTInduced by UVB |
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| DOI： |
| 中文关键词: 补骨脂素 UVB 角质形成细胞 光老化 JNK 信号通路 |
| 英文关键词: Psoralen UVB rays HaCaT Light aging JNK signaling pathway |
| 基金项目:黑龙江省应用技术研究与开发计划项目（PC13S15）；黑龙江中医药大学科研基金项目（201309）；国家自然科学基金面上项目
（81274035）；黑龙江省博士后科研启动基金（LBH-Q13162）；黑龙江省自然科学基金项目（D201231） |
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| 中文摘要: |
| 目的：研究补骨脂素对中波紫外线（UVB）导致人皮肤HaCaT细胞光老化的保护作用及其作用机制。方法：选择不同浓度的
补骨脂素，MTT 法筛选药物的浓度；使用中波紫外线（UVB）照射永生化的HaCaT 细胞建立UVB光老化模型；使用三种不同浓度
的补骨脂素处理光老化模型，MTT 法检测细胞的增殖及氧化试剂盒检测细胞中氧化酶的活性。RT-PCR 及Western Blot 分别检测
JNK 和白介素-8（IL-8）mRNA 及蛋白表达量。结果：与空白组相比，10-7 mol/L、10-6mol/L、10-5mol/L补骨脂素组对HaCaT 具有无
明显的增殖作用（P>0.05）；与模型组相比，10-7 mol/L、10-6mol/L、10-5 mol/L补骨脂素组对HaCaT 具有无明显的增殖作用（P>0.
05），但是10-7 mol/L、10-6mol/L、10-5 mol/L补骨脂素组SOD、GSH、CAT 活性升高（P<0.01），细胞JNK、IL-8 mRNA 表达量均降低
（P<0.01），细胞JNK、IL-8 蛋白表达量均降低（P<0.05 或P<0.01）。结论：补骨脂素能够显著的保护HaCaT 细胞的光老化，其机制
可能与增强抗氧化酶活性，及抑制JNK信号通路，减少炎症因子的分泌有关。 |
| 英文摘要: |
| Objective:To study the protective role of psoralen grain on light aging of human skin HaCaT cells induced by UVB
rays (UVB) and its underlying mechanism.Methods:The different concentrations of psoralen were selected and the suitable
concentration of the drugs was screened by MTT method. Using UVB rays (UVB) radiation, immortalized HaCaT cells UVB light aging
model was established, and then treated with three different concentrations of psoralen processing. The cell activity was determined by
MTT method and oxidase activity in the cell was detected by kits, and the expression of JNK and interleukin 8 (IL-8) mRNA and protein
was respectively detected by RT-PCR and Western Blot.Results:Compared with blank group, 10-7mol/L, 10-6mol/L, 10-5 mol/L psoralen
grain hadn,t obvious effect on the proliferation of HaCaT cells (P>0.05); compared with UVB model group, the cell proliferation in
psoralen group had no significant change (P>0.05), but after treatment with 10-7 mol/L, 10-6 mol/L, 10-5 mol/L psoralen, the activity of
SOD and GSH and CAT was increased(P<0.05), and the expression of JNK and IL-8 mRNA was decreased(P<0.01), and the expression
of JNK and IL-8 protein was also decreased (P<0.05 or P<0.01).Conclusion:The psoralen grain can significantly protect HaCaT cells
light aging, and its mechanism may be related with increasing the antioxidase activity, inhibiting JNK signaling pathways, and reducing
the secretion of inflammatory cytokines. |
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