| 周翠红,顾 婷,何珊珊,李 锐,彭正午,王化宁.异氟烷对小鼠神经干细胞BDNF/Caspase3 及Notch信号相关基因表达的影响[J].,2017,17(1):16-19 |
| 异氟烷对小鼠神经干细胞BDNF/Caspase3 及Notch信号相关基因表达的影响 |
| Effect of Isoflurane on Expression of BDNF/Caspase3 and Notch in Cultured Neural Stem Cells |
| 投稿时间：2016-06-03 修订日期：2016-06-20 |
| DOI：10.13241/j.cnki.pmb.2017.01.004 |
| 中文关键词: 异氟烷 神经干细胞 Notch BDNF |
| 英文关键词: Isoflurane Neural stem cells Notch BDNF |
| 基金项目:国家自然科学基金项目（81401109，81571309） |
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| 中文摘要: |
| 摘要 目的：探讨异氟烷对小鼠神经干细胞的BDNF、Caspase3及Notch信号相关基因表达的影响。方法：给予体外培养的新生小鼠海马神经干细胞不同浓度异氟烷处理，实验分为对照组和异氟烷处理组（ISO1.0，ISO1.5），其中异氟烷组细胞分别给予1.0 MAC和1.5 MAC两个浓度的异氟烷处理2小时，对照组给予O2处理2小时，随后置于培养箱正常培养24小时后收集细胞，提取细胞RNA检测BDNF，Caspase3及Notch相关基因（Notch2、Notch 3和Hes5）的mRNA水平变化。结果：与对照组相比，(1)异氟烷组小鼠神经干细胞的功能基因BDNF mRNA水平下调，凋亡相关基因Caspase3的mRNA水平上调；（2）异氟烷组神经干细胞的Notch2和Notch3受体mRNA表达下调，Notch信号通路靶基因Hes5的mRNA水平也明显下调；（3）异氟烷对神经干细胞的作用具有剂量依赖性，浓度越高对神经干细胞BDNF、Caspase3及Notch信号相关基因表达的影响越大。结论：异氟烷可能通过抑制小鼠神经干细胞的Notch信号通路，下调BDNF的mRNA表达，上调Caspase3的mRNA水平，影响神经干细胞的正常功能。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of isoflurane on the expression of brain derived neurotrophic factor (BDNF), Caspase3 and Notch signaling in neural stem cells. Methods: Cultured hippocampal neural stem cells (NSCs) from newborn mice were divided into control and isoflurane groups: control group was treated with O2 for 2 hours and isoflurane treatment groups (ISO1.0 or ISO1.5) were treated with 1.0 MAC and 1.5 MAC of isoflurane for 2 hours respectively. Then cells were cultured in the incubator with 5% CO2 and 37℃ for 24 hours. And the total RNA was extracted from cells and the mRNA levels of BDNF, Caspase3, Notch2, Notch3, and Hes5 were measured by Real-time PCR. Results: (1) Compared with the control, isoflurane significantly down-regulated the BDNF mRNA level and up-regulated the Caspase3 mRNA level in the neural stem cells; (2) Isoflurane significantly decreased the mRNA levels of Notch2, Notch3 and Hes5 in the neural stem cells; (3) The effect of isoflurane revealed a dose effect---compared with ISO1.0 group, cells treated with 1.5 MAC of isoflurane suffered more influence. Conclusion: Isoflurane exposure suppressed the BDNF expression and in- creased apoptosis of cultured neural stem cells, and this effect possibly mediated by Notch signal pathway. |
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