| 徐芳琴 杜小波 姚敏娜 宁旻 余志 李健康 文爱东.马齿苋水提物对溃疡性结肠炎保护作用的实验研究[J].,2016,16(32):6239-6243 |
| 马齿苋水提物对溃疡性结肠炎保护作用的实验研究 |
| Protective Effects of Aqueous Extract from Portulacaoleracea L.on Ulcerative Colitis |
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| DOI： |
| 中文关键词: 马齿苋 溃疡性结肠炎 氧化应激 炎症 |
| 英文关键词: Ulcerative colitis Oxidative stress Inflammatory |
| 基金项目:国家自然科学基金项目（81373947）；中国新药创新与研发关键技术(2012ZXJ09303011；2012BAK25B00)；
西京医院助推计划基础研究探索项目(XJZT15M20) |
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| 中文摘要: |
| 目的：探讨马齿苋水提物对溃疡性结肠炎（Ulcerative colitis, UC）的保护作用。方法：利用葡聚糖硫酸钠(Dextran sulfate
sodium, DSS)诱导C57BL/6 复制UC模型，将小鼠随机分为6 组，分别为正常组，DSS模型组，SASP 对照组，马齿苋低、中、高剂量
组(100, 200 mg/kg 和400 mg/kg)，每天ig 给药1 次，记录小鼠体重并统计疾病活动指数（Disease activity index, DAI）评分，连续7
天。于第8 天，测定结肠长度，测定MDA、SOD 以及NO含量，观察结肠病理学形态变化，并测定IL-6, IL-1β与TNF-αmRNA的
表达。结果：模型组与正常组比较，小鼠体重，结肠长度及SOD 有显著降低（P<0.05），而DAI 评分，MDA、NO 含量以及TNF-α,
IL-6 与IL-1βmRNA 的表达有明显升高（P<0.05）。而马齿苋水提物中、高组与模型组比较，小鼠的体重，结肠长度以及SOD 含量
均有显著性提高（P<0.05），结肠组织病理损伤有所改善，DAI 评分MDA、NO 含量以及TNF-α, IL-6 与IL-1βmRNA 的表达均有
明显降低（P<0.05）。结论：马齿苋水提物对UC 具有保护作用，可能与改变SOD、NO 与MDA 而抑制氧化应激反应及下调
TNF-α, IL-6与IL-1β等细胞因子mRNA 的表达而降低炎症反应有关。 |
| 英文摘要: |
| Objective:To purslane the protective effects of aqueous extract from Portulacaoleracea L.(AEP) on ulcerative colitis
(UC).Methods:Use the dextran sulfate sodium (DSS) to establish the UC model, the mice of this study were divided into control group,
DSS group, SAPA group (450 mg/kg), and low, medium and high-dose of AEP groups (100 mg/kg, 200 mg/kg and 400 mg/kg). The
animals were given relevant medicine intragastrically once and then weighed and disease activity index (DAI) score were recorded daily
for 7 days. The colon was excised in day 8, measured the length, detect the level of SOD, NO, MDA by ELISA kit, revealed the
morphological features of the colon, and analyze the expression of IL-6, IL-1β and TNF-α.Results:Compared with control group, the
body weight, the level of SOD and the colon length were decreased significantly（P<0.05）, the DAI score, the level of NO, MDA, the
expression of IL-1β, IL-6 and TNF-αmRNA were significantly increased in model group （P<0.05）. The aqueous extract from
groups compared with model group couldimprove the pathologic changes, increase the body weight, the level of
SOD and the colon length, and decrease the DAI score and the level of NO, MDA, and meanwhile the expression of IL-1β, IL-6 and
TNF-αmRNA were also decreased significantly (P<0.05).Conclusion:The AEP show that the protective effects on DSS induced UC
model, and the mechanism may be were inhibit the oxidative stress through MDA, NO, SOD and reduce the inflammatory through the
anti the over-expressions of TNF-α, IL-6 and IL-1β. |
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