| 汪丹丹 王晓敏 唐艳立 王广友 王菁华 张彤帅 王双双 张浩强 徐旭.脑缺血再灌注损伤过程中小胶质细胞向树突状细胞转化的研究[J].,2016,16(30):5843-5846 |
| 脑缺血再灌注损伤过程中小胶质细胞向树突状细胞转化的研究 |
| The Transformation of Microglia into Dendritic Cells during CerebralIschemia Reperfusion Injury |
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| DOI： |
| 中文关键词: 脑缺血再灌注损伤 小胶质细胞 树突状细胞 |
| 英文关键词: CIRI MG DC |
| 基金项目:黑龙江省教育厅科学技术研究项目（12531296） |
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| 中文摘要: |
| 目的：探讨脑缺血再灌注损伤（CIRI）过程中小胶质细胞（MG）向树突状细胞（DC）转化情况；方法：线栓法建立CIRI 小鼠模
型；免疫荧光方法检测MG转化为DC情况；流式细胞仪检测MG 活化情况，MG 转化为DC情况及由MG 转化而来的DC 表达
MHC-II情况；结果：CIRI过程中MG（CD11b+）表达DC（CD11c+）表面标志；与Sham 组相比，不同时间点CIRI小鼠脑组织中，活
化的MG（CD11b+CD45med）显著增多（P1d<0.01，2 d、4 d、6 d组P<0.001），活化的MG 转化为DC（CD11b+CD45medCD11c+）的
数量显著增多（P<0.001），4 d 时达到高峰，4 d组与1 d、2 d、6 d组相比显著增多（P<0.001，P<0.01，P<0.05），并且，由MG转化而来
的DC 表达MHC-II显著增多（P<0.001），4 d组与1 d、2 d、6 d组相比，CD11b+CD45medCD11c+MHC-II+细胞数量显著增多（P<0.
001，P<0.001，P<0.05）；结论：CIRI过程中MG 能够转化为DC，并且，由MG转化而来的DC 具有抗原递呈作用。 |
| 英文摘要: |
| Objective:To investigate the transformation of microglia (MG) to dendritic cells (DC) in the process of cerebral
ischemia reperfusion injury (CIRI).Methods:CIRI mouse model was established by thread embolism method. Immunofluorescence
staining was used to detect the transformation of MG into DC. Flow cytometry was used to detect the activation of MG, transformation of
MG into DC as well as the MHC-II expression on DC transformed from the MG.Results:MG (CD11b+) expression DC (CD11c+)
surface markers during CIRI. Compared with Sham group, activated MG (CD11b+CD45med) increased significantly（P1d<0.01, 2d, 4d,
6d group P<0.001）, the number of activated MG transformed into DC (CD11b+CD45medCD11c+) was significantly increased (P<0.
001), which reached its peak at day 4, and was significantly increased compared with 1d, 2d, 6d group (P<0.001, P<0.01, P<0.05) in
brain tissue of CIRI mice. The expression of MHC-II was significantly increased (P<0.001) in the DC transformed from MG.
CD11b+CD45medCD11c+MHC-II+ cell number was significantly increased in 4d group compared with 1 d, 2 d, 6 d group (P<0.001,
P<0.001, P<0.05).Conclusion:MG can transforminto DC during CIRI, this kind of DC has the function of antigen presentation. |
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