| 卢颖 孙磊 王庆丽 高堃 宋成伟.BML-111 对糖尿病大鼠心肌炎症反应及细胞凋亡的影响[J].,2016,16(29):5620-5624 |
| BML-111 对糖尿病大鼠心肌炎症反应及细胞凋亡的影响 |
| Effects of BML-111 on Inflammation and Apoptosis of Cardiomyocytes inDiabetic Rats |
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| DOI： |
| 中文关键词: BML-111 糖尿病 细胞凋亡 炎症反应 |
| 英文关键词: BML-111 Diabetes mellitus Apoptosis Inflammation |
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| 中文摘要: |
| 目的：探讨BML-111 治疗对糖尿病大鼠心肌细胞凋亡及炎症反应的影响。方法：33 只雄性Wistar 大鼠随机分为对照
（Control）组、糖尿病（DM）组和BML-111 组，每组各11 只。Control组喂以普通饲料，而其它两组大鼠喂以高糖高脂饲料。饲养6
周后，DM 组及BML-111 组大鼠分别腹腔注射链尿佐菌素30 mg/kg，并于1 周后建立糖尿病模型。模型制备完成后12 周，
BML-111 组大鼠每日腹腔注射BML-111 0.5 mg/kg，其它两组大鼠腹腔注射相同容量生理盐水。12 周后，测定体重、甘油三酯
（TC）、总胆固醇（TG）水平、空腹血糖，采用TUNEL法测定心肌细胞凋亡指数（AI），Western blot 法测定IL-6、TNF-alpha蛋白表达水
平，并使用试剂盒测定心肌SOD 活性和MDA 含量。结果：与Control 组比较，DM组大鼠体重显著下降，TC、TG、空腹血糖水平明
显升高（P<0.05）；与DM 组比较，BML-111 组大鼠体重增加，甘油三酯、总胆固醇、空腹血糖水平下降（P<0.05）。与Control 组比
较，DM组大鼠心肌凋亡指数显著增加（P<0.05）；而与DM 组比较，BML-111 组大鼠心肌凋亡指数显著降低（P<0.05）。与Control
组比较，DM 组大鼠心肌IL-6、TNF-α、p-NF-κB、p-p38MAPK 蛋白表达水平及MDA 含量明显增高（P<0.05），而SOD 活性降低
（P<0.05）；与DM 组比较，BML-111 组大鼠心肌IL-6、TNF-α、p-NF-κB、p-p38MAPK蛋白表达水平及MDA含量较低（P<0.05），而
SOD 活性增加（P<0.05）。结论：BML-111 可减轻糖尿病大鼠心肌炎症反应及细胞凋亡。 |
| 英文摘要: |
| Objective:To study effects of BML-111 on inflammation and apoptosis of cardiomyocytes in diabetic rats.Methods:Thirty-three male Wistar rats were randomly divided into three groups including Control group (n=11), Diabetes mellitus (DM) group
(n=11) and BML-111 group (n=11). The experimental diabetic rat model was induced by a dose of streptozoticin (STZ, 30 mg/kg) combined
with high energy intake on rats. Rats in Control group were fed with common diet while those in the other two groups were fed
with high fat diet. One week after the build-up of the model, rats in BML-111 group received the daily intraperitoneal injection of 0.5
mg/kg of BML-111 for 12 weeks, while those in the other two groups received the same volume of saline intraperitoneally. Twelve weeks
later, the body weight, fasting blood glucose, triglyceride (TG) and total cholesterol (TC) were detected; Apoptosis was examined by Terminal
deoxynucleotidyl transferase dUTP nick end labeling (TUNEL); The protein expressions of IL-6, TNF-α, phospho-NF-κB and
phospho-p38MAPK were detected by western blot analysis; The activity of Superoxide dismutase (SOD) and the content of Malondialdehyde
(MDA) were detected with commercial kits.Results:Compared with Control group, the body weight was lower and the levels of
fasting blood glucose, TG and TC were higher in DMgroup (P<0.05). Compared with DM group, the body weight was higher and the
levels of fasting blood glucose, TG and TC were lower in BML-111 group (P<0.05). Compared with Control group, the apoptotic index
(AI), the protein expressions of IL-6, TNF-α, phospho-NF-κB, phospho-p38MAPK and the content of MDA were higher whereas the activity
of SOD was lower in the myocardial tissue of rats in BML-111 group (P<0.05); Compared with DMgroup, the apoptotic index, the
protein expressions of IL-6 and TNF-琢, the content of MDA were lower whereas the activity of SOD was higher in the myocardial tissue
of rats in BML-111 group (P<0.05).Conclusion:BML-111 treatments could attenuate inflammation and apoptosis in cardiomyocytes of
diabetic rats. |
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