| 杨奕 孟松艳 李玛丽 迟啸威 滕宗艳 李祥禄 于卫刚.外周血单核细胞端粒长度和端粒酶hTERT 活性在心力衰竭中的变化研究[J].,2016,16(27):5377-5379 |
| 外周血单核细胞端粒长度和端粒酶hTERT 活性在心力衰竭中的变化研究 |
| Changes of Telomerase hTERT Activity and Telomere Length of Peripheralblood Mononuclear Cell in Heart Failure |
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| DOI： |
| 中文关键词: 心力衰竭 端粒 端粒酶 |
| 英文关键词: Heart Failure Telomere Telomerase |
| 基金项目:黑龙江省教育厅科学技术研究面上项目(12531423) |
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| 中文摘要: |
| 目的：探讨心力衰竭患者外周血单核细胞端粒长度和端粒酶hTERT 活性在心衰发生进程中的变化情况和意义。方法：按照
筛选要求选择患者，根据入选标准分为心衰组(49例)和非心衰组(44例)。记录患者的年龄、性别、生活习惯及疾病情况，超声检测
患者心脏功能，测量左心室舒张末内径(LVEDD)、左心室射血分数(LVEF)。在不同时间点，抽取外周血分离单核细胞，用PCR 方
法检测端粒长度和端粒酶hTERT 活性。结果：对照组比较，心衰组患者心脏左室舒张末内径明显增加，射血分数明显降低(P<0.
05)；在第1、7 天，心衰组患者外周血单核细胞端粒长度较对照组明显缩短、端粒酶hTERT 活性明显增强。第7 天较同组第1天端
粒长度有所增加，端粒酶hTERT 活性有所减低，但与对照组相比，端粒长度显著缩短，端粒酶hTERT 显著增高(P<0.05)。结论：心
力衰竭后患者端粒长度和端粒酶hTERT 活性明显变化，并随心衰发病具有一定波动，提示端粒和端粒酶可能参与了心衰发展进
程。 |
| 英文摘要: |
| Objective:To investigate the changes of telomerase hTERT activity and telomere length of Peripheral blood mononuclear
cell in patients with heart failure.Methods:49 heart failure patients and 44 healthy people were divided into two groups: heart failure
group (HFG) and the control group (CG). Make the record of patients' general conditions. Take the ultrasonic testing of left ventricular
end-diastolic dimension and left ventricular ejection fraction. Isolate the monocytes of blood collection at different time points. The
levels of telomere length and telomerase hTERT activity in each group was estimated using real-time fluorescent quantitative PCR
(RT-PCR).Results:The LVEF of HFG showed obvious reduction (P<0.05)compared with that of CG. The patients of HFG showed low
levels of telomere length but high levels of telomerase hTERT activity after the diagnose of heart failure. The telomere length of HFG in
7d increased obviously compared with that in 1d (P<0.001). The telomerase hTERT activity of HFG in 7 d is lower than that in 1 d, but
they both increased obviously (P<0.05) compared with those of CG.Conclusion:Telomere length and telomerase hTERT activity of peripheral
blood mononuclear cells in patients of HFG have significant differences compared with those of CG. They both fluctuated during
the development course of the disease，which showed that telomere length and telomerase hTERT activity of peripheral blood
mononuclear cells are playing important role in the disease of heart failure. |
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