| 许可嘉 张天娇 韩森 张玮 赵福建 李健△.细胞自噬参与雷公藤甲素诱导的肝细胞损伤[J].,2016,16(26):5012-5014 |
| 细胞自噬参与雷公藤甲素诱导的肝细胞损伤 |
| Autophagy Involved in Hepatic Injury Induced by Triptolide |
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| DOI： |
| 中文关键词: 自噬 雷公藤甲素 肝损伤 |
| 英文关键词: Autophagy Triptolide Hepatic injury |
| 基金项目:国家自然科学基金项目（81273884） |
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| 中文摘要: |
| 目的：观察雷公藤甲素引起急性肝损伤时肝细胞自噬的情况。方法：采用LC3-GFP 质粒尾静脉高压注射的方法在体监测雷
公藤甲素诱导肝细胞自噬的发生；用腺病毒双荧光载体mRFP-GFP-LC3 感染体外培养的HepG2 细胞，通过激光共聚焦显微镜和
蛋白印记的方法检测细胞自噬流的强度。结果：小鼠腹腔注射雷公藤甲素(0.25 mg/kg 和0.5 mg/kg)24 小时，血清中ALT 和AST
水平均显著升高；肝组织病理结果显示肝细胞肿胀、空泡化及坏死；激光共聚焦显微镜下可检测到LC3-GFP 质粒表达所呈现的绿
色荧光；离体细胞实验结果证实雷公藤甲素可诱导肝细胞发生自噬流，且表现为一定程度的剂量、时间依赖性。结论：雷公藤甲素
可引发急性肝损伤并伴随明显的细胞自噬。 |
| 英文摘要: |
| Objective:To observe the characteristics of hepatocyte autophagy in triptolide induced acute liver injure.Methods:To
monitor the relationship between hepatocyte autophagy and triptolide induced liver injure in vivo by using the tail vein injected LC3-GFP
plasmid; To monitor the autophagy flux strength of dual fluorescence mRFP-GFP-LC3 adenovirus vector infected HepG2 cells in vitro by
using confocal laser scanning microscopy and Western blot.Results:After mice were administrated triptolide by intraperitoneal injection
(0.25 mg / kg and 0.5 mg / kg) 24 hours, the ALT and AST in serum were significantly elevated; Liver tissue pathology results show that
liver cells revealed swelling, vacuolization and necrosis; LC3-GFP expressed green fluorescence of plasmid was detected by confocal
laser scanning microscopy; The results of cell experiments in vitro showed that triptolide can induce autophagy flux of liver cells in dose
and time dependent manner.Conclusion:Triptolide can induce acute hepatic injury with cell autophagy. |
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