| 陶颖 徐明新 Jebun Nessa Diana 陈园园 袁伟恩.胶原酶缓释微球的研究[J].,2016,16(25):4826-4828 |
| 胶原酶缓释微球的研究 |
| Research on Collagenase-loaded Microspheres |
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| DOI： |
| 中文关键词: 胶原酶 缓释 微球 释放 |
| 英文关键词: Collagenase Sustained release Microsphere Release |
| 基金项目:国家自然科学基金项目（81373366）；上海交通大学医工交叉项目（YG2013MS52，YG2013MS62） |
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| 中文摘要: |
| 目的：本实验旨在开发一种胶原酶缓释微球制剂，用以治疗手掌腱膜挛缩症，以减小现有水针剂的不足。方法：利用水相-水
相乳化法和低温冷冻相分离法两种方法制备载药颗粒，分别将其包裹于PLGA 微球内，制备成胶原酶微球，并用扫描电镜考察其
表面形态，对其粒径进行统计学分析，测定体外释放行为并比较。结果：两种方法制备的微球表面光滑圆整，都可以达到缓释的效
果，一个星期内释药完全。水相-水相乳化法制备的微球比低温冷冻相分离制备的微球粒径大，且具有统计学差异（P<0.05）。水相
-水相乳化法制备的微球粒径较均一，其体外释放更加平缓，突释较小。结论：本研究制得的胶原酶微球能实现理想的体外缓释效
果，解决了现有技术中胶原酶粉针剂型快速释放并分散的问题。 |
| 英文摘要: |
| Objective:To research the sustained release of collagenase-loaded microspheres to treat Dupuytren's disease, as well as
to compensate the shortcoming of powder injection.Methods:Aqueous-aqueous emulsion method and freezing phase separation method
were used to make micro-particles respectively. Then the particles were encapsulated into PLGA microspheres. The microspheres were
observed by SEM. Statistical analysis of the sizes were made between the two methods. And in vitro release curves of microspheres were
tested to compare the two methods.Results:The microspheres prepared by the two different methods were spherical. Sustained release of
microspheres by two different ways could be achieved. The sizes of microspheres by aqueous-aqueous emulsion method were significantly
larger than that of the freezing phase separation method (P<0.05). The release of collagenase fromthe microspheres by aqueous-aqueous
emulsion method was more moderate with low burst release.Conclusion:Collagenase microspheres would achieve sustained release for
one week to solve the problemof fast drug diffusion after powder injection. |
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