| 吕卫东 张家东 张新伟 雷光焰 蔡琳 刘志刚 陆建荣.小鼠去细胞拟胚体对小鼠Lewis肺癌细胞体内生长的影响[J].,2016,16(25):4822-4825 |
| 小鼠去细胞拟胚体对小鼠Lewis肺癌细胞体内生长的影响 |
| Effect of Acellular Mouse Embryoid Bodies on Growth of Mouse Lewis LungCancer Cells in Vivo |
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| DOI： |
| 中文关键词: 胚胎干细胞 去细胞 拟胚体 细胞外基质 生物材料 |
| 英文关键词: Embryonic stemcell Decellularization Embryoid bodies Extracellular matrix Biomaterials |
| 基金项目:国家自然科学基金项目（31100686）；陕西省自然科学基金面上项目（2015JM8435） |
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| 中文摘要: |
| 目的：阐明小鼠去细胞拟胚体对小鼠Lewis 肺癌细胞在体内生长的影响。方法：先制备来源于小鼠胚胎干细胞的拟胚体，然
后用SDS 去细胞处理。实验分成3 组：小鼠Lewis 肺癌细胞与去细胞拟胚体培养组，癌细胞与Matrigel 培养组和单纯癌细胞组
（每组n=12）。培养3 天后注射入裸鼠体内，观察肿瘤生长情况。28 天取出瘤体，Ki67和CD31 免疫组化染色（n=12）检测细胞增殖
和肿瘤微血管密度(MVD)，Western blot 检测组织Paxillin，E-cadherin 和茁-actin 水平（n=6）。结果：去细胞拟胚体组肿瘤生长明显
较单纯细胞组和Matrigel 组慢。去细胞拟胚体组Ki67 指数（（17.1± 2.6）%）明显小于单纯细胞组（（34.5± 4.7）%）和Matrigel 组
（（48.4± 8.6）%）（P<0.05）；去细胞拟胚体组的MVD（18.7± 3.6 个/mm2）明显小于单纯细胞组（32.1± 6.4 个/mm2）和Matrigel 组
（42.6± 7.1 个/mm2）（P<0.05）。Western blot 结果提示去细胞拟胚体组的Paxillin 表达小于单纯细胞组和Matrigel 组（P<0.05），而
E-cadherin 表达大于单纯细胞组和Matrigel 组（P<0.05）。结论：小鼠去细胞拟胚体对小鼠Lewis 肺癌细胞在体内有明显的促分化
作用。 |
| 英文摘要: |
| Objective:To investigate the effect of mouse acellular embryoid bodies (EBs) on growth of mouse Lewis lung cancer
cells in vivo.Methods:Mouse acellular EBs were prepared by decellularization of mouse EBs with sodium dodecyl sulfate (SDS). Mouse
Lewis lung cancer cells were seeded in the acellular EBs, with cultured in Matrigel and cultured at 2-dimension as controls (n=12 for
each group). After cultured for 3 days, cells were injected to 6-week old BALB/c (nu/nu) mice for subcutaneous tumor experiment. Tumors
were retrieved at 28-day implantation. Immunohistochemical staining (n=12) and western blot (n=6) were used to evaluate cell proliferation,
microvessel density and protein expression levels.Results:The tumor volumes of acellular EBs group were less than those of
Matrigel group and 2-dimension control group (both P<0.05). Immunohistochemical staining showed Ki67 labeling index and microvessel
density labeled by CD31 less than Matrigel group and 2-dimension control group (all P<0.05). Western blot presented the least Paxillin
and the most E-cadherin expression for acellular EBs group.Conclusion:The acellular EBs promote differentiation of mouse Lewis
lung cancer cells in vivo. |
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