| 白马恒 韩康 周勇 刘增亮 邢永军.miRNA-194 对人骨肉瘤细胞系SOSP-9607 转移的作用研究[J].,2016,16(23):4423-4426 |
| miRNA-194 对人骨肉瘤细胞系SOSP-9607 转移的作用研究 |
| Effects of MiR-194 on Metastasis of Human Osteosarcoma Cell LineSOSP-9607 |
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| DOI： |
| 中文关键词: 微小RNA 慢病毒 骨肉瘤 转移 miRNA-194 |
| 英文关键词: miRNA Lentivirus Osteosarcoma Metastasis MiRNA-194 |
| 基金项目:国家自然科学基金项目（81201633）；院长基金项目（2015ZX01） |
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| 中文摘要: |
| 目的：通过建立表达不同高低水平miRNA-194 的骨肉瘤细胞系，研究和探测miRNA-194 对于骨肉瘤细胞转移特性的影响
和作用。为进一步研究miRNA-194 作为生物治疗的新靶点提供理论依据。方法：使用慢病毒技术，对骨肉瘤细胞进行转染和筛
选，获得表达不同高低水平miRNA-194 的骨肉瘤细胞系并进行分组。通过Transwell 实验，划痕实验对miRNA-194 在骨肉瘤中的
作用进行探索。结果：1）慢病毒转染及筛选成功，获得表达不同高低水平miRNA-194 的骨肉瘤细胞系；2）transwell 迁移及侵袭实
验中，miRNA-194过表达组的迁移（28.60± 4.36）及侵袭（21.25± 6.42）能力都显著的小于其余各组，相应的，miRNA-194 沉默表达
组（132.60± 15.64；115.76± 11.38）则高于其余各组。划痕实验结果显示，miRNA能够显著的抑制骨肉瘤细胞SOSP-9607 的划痕
愈合能力（P<0.01）。结论：miR-194 能够对骨肉瘤细胞SOSP-9607 的转移起到明确的抑制作用。MiRNA-194 有望成为骨肉瘤转移
与治疗的新靶点。 |
| 英文摘要: |
| Objective:miRNA-194 were knocked down or overexpressed in Osteosarcoma Cell Line SOSP-9607 to investigate its
effect on Metastasis of human Osteosarcoma which to provide the foundation of theory of miRNA-194 as a new target of osteosarcoma
therapy.Methods:We transfected human osteosarcoma cell lines SOSP-9607 by using recombinant lentiviruses and divided the cells into
three groups with different levels of miRNA-194. Then the stable transfected cells were used in transwell and wound healing assay.Results:1) The transfection and selection of miRNA-194 using recombinant lentiviral expression vector was finished correctly and different
levels of miRNA-194 were got sucessfully. 2) Results in the transwell migration and invasion assay showed significantly lower numbers
of OE SOSP-9607 cells (28.60± 4.36) and (21.25± 6.42) and significantly higher numbers of KD SOSP-9607 cells (132.60± 15.64;
115.76± 11.38) compared with other groups (p<0.05). The wound healing assay showed that cells in OE groups exhibited a significant
decrease in migration rate compared to the other three groups(p<0.05).Conclusion:miRNA-194 could inhibit metastasis of Osteosarcoma
Cell Line SOSP-9607. MiRNA-194 could be further explored as a potential target in Osteosarcoma diagnosis and therapy. |
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