| 吴国志 欧积壮 吴昌新 林维 刘圣星 王卫国.巴戟天及其糖提取物对于体外培养成骨细胞OPG/RANKL基因系统表达的影响[J].,2016,16(20):3849-3852 |
| 巴戟天及其糖提取物对于体外培养成骨细胞OPG/RANKL基因系统表达的影响 |
| Effect of Morinda Root and its Polysaccharide Extract on OPG / RANKL Gene Expression of in vitro Osteoblast |
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| DOI： |
| 中文关键词: 巴戟天 骨保护素 核因子kB 受体活化因子配体 骨质疏松 |
| 英文关键词: Morinda OPG Receptor activator of nuclear factor kB ligand Osteoporosis |
| 基金项目:海南省卫计委一般科研项目（14A200077） |
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| 中文摘要: |
| 目的：探讨巴戟天及多糖提取物对成骨细胞骨保护素（OPG）／核因子资B 受体活化因子配体（RANKL）基因系统表达的影
响。方法：取2~3天的SD 大鼠5 只分离原代成骨细胞，再取8 周龄SD 大鼠35 只随机分为七组，对照组不进行处理，三组给予
10 g/L、50 g/L、100 g/L巴戟天水灌胃，其余三组分别给予10 g/L、50 g/L、100 g/L巴戟天多糖灌胃，72 h后采用采用ELISA法测定
培养液中OPG、RANKL及骨钙素的含量，采用MTT 法检测不同浓度巴戟天水及多糖提取物对大鼠成骨细胞增殖的影响，采用荧
光定量PCR 检测OPG 和RANKL mRNA表达情况；通过Western blot检测OPG和RANKL蛋白表达水平。结果：巴戟天水及多
糖提取物组A570 nm、ALP 活性、骨钙素含量、OPG/RANKL mRNA 表达量、OPG 和RANKL蛋白表达阳性密度均高于对照组
（P＜0.05）；A570 nm、ALP 活性、骨钙素含量、OPG/RANKL mRNA 表达量、OPG 和RANKL 蛋白表达阳性密度均高于同等剂量的
水提取物各组（P＜ 0.05)；巴戟天多糖组中随着多糖剂量的升高A570 nm、ALP 活性、骨钙素含量、OPG/RANKL mRNA 表达量、
OPG 和RANKL蛋白表达阳性密度，差异比较有统计学意义（P＜0.05）。结论：巴戟天水及多糖提取物均能促进体外培养成骨细胞
的增殖，提高成骨细胞活性。 |
| 英文摘要: |
| Objective:To investigate effect of Morinda root polysaccharide and its extract on OPG/RANKL gene expression of in
vitro osteoblast, so as to make further research on molecular mechanisms of Morinda in the treatment of osteoporosis.Methods:Take 5
SD rats birth from 2 to 3 days to separate primary osteoblasts, and then take 35 SD rats 8 weeks old randomly divided into 7 groups. The
control group did not take treatment. Three polysaccharide groups were given separately 10 g / L, 50 g / L, 100 g / L Morinda water
gavage, and the remain three groups were given separately 10 g / L, 50 g / L, 100 g / L Morinda polysaccharide gavage. After 72 h, the
ELISA assay was used to detect the contents of broth OPG, RANKL and bone calcium. MTT was applied to investigate the effect of
different concentrations of Morinda officinalis water and polysaccharide extract on the proliferation of rat bone. And Real-time PCR was
used to detect the mRNA expression of OPG and RANKL. Western blot was applied to analyze the protein levels of OPG and RANKL.Results:In the Morinda officinalis water and polysaccharide extract groups, the A570 nm, ALP activity, osteocalcin content, OPG /
RANKL mRNA expression, OPG and RANKL protein expression density were higher than in control group (P <0.05). The A570 nm,
ALP activity, osteocalcin content, OPG / RANKL mRNA expression, OPG and RANKL protein expression density were also higher than
in each group of the same dose of water extract (P<0.05). In the Morinda officinalis polysaccharide groups, along with the increasing
doses of polysaccharide, A570 nm, ALP activity, osteocalcin content, OPG / RANKL mRNA expression, OPG and RANKL protein
expression density all had statistically significant difference (P<0.05).Conclusion:The Morinda officinalis and its polysaccharide extract
can promote the in vitro proliferation of osteoblast cell, and enhance osteoblast activity. |
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