| 王旭杰 丘瑾 蔡蓓珺 季寒冰 刘英姿.变异性分化抗原簇44（CD44v17）对宫颈癌的临床诊断意义[J].,2016,16(19):3625-3628 |
| 变异性分化抗原簇44（CD44v17）对宫颈癌的临床诊断意义 |
| Clinical Diagnosis Value of CD44V17 in Cervical Cancer |
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| DOI： |
| 中文关键词: 变异性分化抗原簇44 宫颈上皮内瘤变 宫颈癌 |
| 英文关键词: CD44V17 Cervical intraepithelial neoplasia Cervical cancer |
| 基金项目:上海市卫生局科研基金项目（03.02.12.003） |
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| 中文摘要: |
| 目的：探讨CD44v17 对宫颈癌的临床诊断意义。方法：将CD44v17siRNA、CD44v17、生理盐水转染至传代后的人宫颈癌细
胞。检测细胞转染后存活率；检测细胞凋亡率。在裸鼠左肩背部注入人宫颈癌细胞悬液，随机分为CD44v17 组、CD44v17siRNA
组、对照组。在CD44v17 组、CD44v17siRNA 组裸鼠瘤体内分别注入CD44v17 病毒颗粒、CD44v17siRNA病毒颗粒。检测瘤体的质
量与体积。选取疑有宫颈病变患者阴道镜下活检组织80 例，正常宫颈组织15 例、宫颈上皮内瘤变（CIN）I级组织l5 例、CIN II级
15 例、CIN III级组织15 例和宫颈癌组织20 例。检测CD44v17 在不同组织中的表达量。结果：CD44v17siRNA转染的宫颈癌细胞
凋亡率（19.20± 2.14 %）高于CD44v17 转染的宫颈癌细胞凋亡率（6.13± 1.08 %）（P<0.05）。CD44v17 组裸鼠瘤体质量（15.9± 3.4）
g 高于对照组裸鼠瘤体质量(11.8± 2.7)g（P<0.05）。CD44v17 在不同组织中的表达量，按正常宫颈、CIN Ⅰ级、CIN Ⅱ级、CIN Ⅲ级、
宫颈癌发展过程呈递增趋势（P<0.05）。结论：CD44v17 能抑制宫颈癌细胞凋亡，促进宫颈癌细胞的生长、增殖。通过降低CD44v17
表达量可能是遏制CIN 向宫颈癌发展的一个手段。 |
| 英文摘要: |
| Objective:To explore the clinical diagnosis value of CD44V17 in cervical cancer.Methods:CD44v17siRNA, CD44v17,
physiological saline was transfected into cultured human cervical cancer cells. The cell viability was detected. The rate of apoptosis was
detected. Human cervical cancer cell was suspensioned the left shoulder of nude mice. The nude mice were randomly divided into
CD44v17 group, CD44v17siRNA group and control group. The nude mice of CD44v17 group and CD44v17siRNA group were injected
with CD44v17 virus particles and CD44v17siRNA virus particles, respectively. The quality and volume of tumor were detected. The
biopsy tissues of totally 80 patients with cervical lesions were selected. According to pathological classification, there were 15 cases of
normal cervical tissues, cervical intraepithelial neoplasia (CIN) I grade 15 cases, II grade CIN 15 cases, III grade CIN 15 cases and 20
cases of cervical cancer tissues. The expression of CD44v17 in different tissues was detected.Results:The rate of apoptosis of cervical
cancer cells transfected with CD44v17siRNA (19.20± 2.14 %) was higher than that of CD44v17 transfected cervical cancer cell (6.13±
1.08 %) (P< 0.05). The quality of tumor (15.9± 3.4) g in CD44v17 group was higher than that in control group (11.8 ± 2.7) g (P<0.05).
The expression of CD44v17 in normal cervix, cervical intraepithelial neoplasia grade, grade II CIN, cervical intraepithelial neoplasia
grade, cervical cancer was progressively increased with statistical significance (P<0.05).Conclusion:CD44v17 can inhibit the apoptosis
of cervical cancer cells and promote the growth and proliferation of cervical cancer cells. By reducing the expression of CD44v17 may be
a effective method to curb the development of CIN to cervical cancer. |
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