文章摘要
张一恺 徐慧 尚沛津 刘天龙 丁一 朱志会 王明明 李玉文 文爱东.alpha- 乳香酸联用羟基红花黄色素A抗胃溃疡效果研究[J].,2016,16(17):3232-3236
alpha- 乳香酸联用羟基红花黄色素A抗胃溃疡效果研究
Gastro-protective Effects of the Combination of alpha-Boswellic Acid and HSYAon Ethanol-induced Gastric Injury in Rats
  
DOI:
中文关键词: alpha-乳香酸  羟基红花黄色素A  胃溃疡  抗炎
英文关键词: alpha-Boswellic Acid  Hydroxysafflor Yellow A  Gastric ulcer  Anti-inflammatory
基金项目:国家自然科学基金项目(81373947;81201985)
作者单位
张一恺 徐慧 尚沛津 刘天龙 丁一 朱志会 王明明 李玉文 文爱东 第四军医大学西京医院药剂科沈阳军区总医院药剂科 陕西省结核病防治院 
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中文摘要:
      目的:研究乳香红花提取物中主要药效物质alpha-乳香酸(alpha-BA)和羟基红花黄色素A(HSYA)联合应用的胃保护效果。方法: 36 只SD 大鼠随机分为6 组:正常组(生理盐水5 mL/kg),模型组(生理盐水5 mL/kg),西咪替丁组(100 mg/kg),HSYA 组(100 mg/kg),alpha-BA 组(200 mg/kg),联合用药组(HSYA 50 mg/kg+alpha-BA 100 mg/kg)。预给药1 小时后,用乙醇诱导SD 大鼠胃溃疡造 模,评价溃疡指数、胃内容物酸度、胃壁黏液量,用ELISA法检测血清炎性因子TNF-alpha、IL-1beta、IL-8。结果:①与造模组相比,alpha-BA 组和联合用药组显著降低乙醇诱导胃粘膜损伤(P<0.001)且联合用药组较西咪替丁组相比差异无统计学意义,但HSYA组差异 无统计学意义;与造模组相比,alpha-BA组和联合用药组显著升高胃内容物pH,增加胃黏液含量(P<0.001)且联合用药组与西咪替 丁组相比差异无统计学意义,但HSYA 组仅胃黏液含量有一定升高(P<0.05),胃内容物pH 没有明显改变。②与造模组相比, HSYA 组、alpha-BA组、联合用药组均显著抑制血清TNF-alpha、IL-1beta、IL-8 的升高(P<0.001)且联合用药组与西咪替丁组相比差异无统 计学意义。③H&E 染色观察可见HSYA组依然有明显出血性损伤;西咪替丁组、琢-BA组和联合用药组则明显保护胃黏膜、抑制 损伤和白细胞浸润。结论:alpha-BA有较好的胃保护效果,HSYA胃保护效果显著低于alpha-BA,但联合应用HSYA、alpha-BA能够抑制胃 黏膜出血、白细胞浸润、抑制胃内容物酸度,增加胃黏液含量,并且能够调节血清炎性因子从而发挥较单用药用量更少且效果更 好的胃保护作用。
英文摘要:
      Objective:To investigate the protective effects of alpha-Boswellic Acid and HSYA on ethanol-induced gastric injury in rats.Methods:All the 36 rats were divided evenly and randomly into the following 6 groups: control group (5 mL/kg physiological saline), model group (5 mL/kg physiological saline), cimetidine group (100 mg/kg), HSYA group (100 mg/kg), alpha-BA group (200 mg/kg), combination group (HSYA 50 mg/kg+alpha-BA 100 mg/kg). One hour before administration of absolute ethanol, the vehicle, cimetidine and various doses of HSYA and alpha-BA were given to each experimental group through administration by gavage. The gastro-protection of effects was assessed by measuring Ulcer Index (UI), gastric juice acidity (pH), gastric wall mucus (GWM) and the level of serum TNF-alpha, IL-1 beta and IL-8 by ELISA.Results:① Compared with model group, UI was decreased significantly in 琢-BA group and combination group (P<0.001) and the differences between cimetidine group and combination group were not statistical significance, while the difference between HSYA group and model group were not statistical significance; Compared with model group, and the pH and GWM were increased markedly in 琢-BA group and combination group (P<0.001) and the differences between cimetidine group and combination group were not statistical significance, however, difference between HSYA group and model group were only statistical significance in GWM (P<0.05). ② Compared with control group, the level of serum TNF-alpha, IL-1betaand IL-8 were significantly in- creased in HSYA group (P<0.05), alpha-BA group and combination group (P<0.001) and the differences between cimetidine group and combination group were not statistical significance. ③ For H&E staining, cimetidine group, alpha-BA group and combination group protected the gastric tissues from hemorrhagic lesions along with attenuation of leukocyte infiltration and submucosal edema.Conclusion:The gastroprotective effects of alpha-BA was better than HSYA, and combination of 琢-BA and HSYA may protect gastric fromhemorrhagic lesions along with attenuation of leukocyte infiltration and submucosal edema through mediating serum TNF-alpha, IL-1beta, IL-8 and gastric juice acidity and gastric wall mucus.
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