| 李楠 叶明翔 张信信 常宁 张艰△.Mcl-1 调控NSCLC 对EGFR-TKIs敏感性的实验研究[J].,2016,16(16):3028-3031 |
| Mcl-1 调控NSCLC 对EGFR-TKIs敏感性的实验研究 |
| Regulation of Mcl-1 on the Sensitivity to EGFR-TKIs of Non-small Cell Lung Cancer |
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| DOI： |
| 中文关键词: 非小细胞肺癌 人髓细胞白血病基因-1 细胞凋亡 |
| 英文关键词: Non-small cell lung cancer Mcl-1 Apoptosis |
| 基金项目:国家自然科学基金项目（81272518） |
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| 摘要点击次数: 955 |
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| 中文摘要: |
| 目的：探讨人髓细胞白血病基因-1(myeloid cell leukemia-1，Mcl-1)是否参与调控非小细胞肺癌(non-small cell lung cancer，
NSCLC)对EGFR-TKIs 的敏感性，为非小细胞肺癌的治疗提供新的思路。方法：通过Western blot 方法检测EGFR-TKIs敏感细胞
H3255 和耐药细胞H1975 中Mcl-1 蛋白的表达水平。分别给予敏感细胞H3255 和耐药细胞H1975 EGFR-TKIs 处理后检测细胞
凋亡情况和Mcl-1蛋白表达水平。设计并合成特异性siRNA 下调耐药细胞H1975 中Mcl-1 的表达，采用脂质体转染后通过流式
细胞技术检测细胞的凋亡情况。结果：H3255 细胞Mcl-1 表达水平明显低于H1975 细胞。一代EGFR-TKIs Gefitinib 显著降低
H3255 细胞Mcl-1 表达而不能减少H1975 细胞Mcl-1 表达。H1975 细胞经二代EGFR-TKIs Afatinib和三代EGFR-TKIs AZD9291
处理后Mcl-1 表达明显减少。特异性siRNA下调H1975 细胞Mcl-1 表达可以促进细胞凋亡。结论：Mcl-1 参与了调节NSCLC 对
EGFR-TKIs的敏感性，可能成为防止或逆转NSCLC 对EGFR-TKIs 耐药的潜在靶点。 |
| 英文摘要: |
| Objective:To explore whether myeloid cell leukemia-1 (Mcl-1) participated in regulating the sensitivity to epidermal
growth factor receptor tyrosine kinase inhibitors of non-small cell lung cancer, and provide new ideas for the treatment of non -small cell
lung cancer (NSCLC).Methods:The expression levels of Mcl-1 in EGFR-TKIs sensitive cell H3255 and resistant cell H1975 were
detected by western blot. Then the expression level of Mcl-1 and apoptosis of sensitive cell H3255 and resistant cell H1975 after being
treated with EGFR-TKIs were examined. The specific siRNA that could down-regulate the expression of Mcl-1 in resistant cell H1975
was designed and synthesized, and the apoptosis of these cells wer detected by flow cytometry after liposome transfection.Results:The
expression of Mcl-1 in H3255 cell was significantly lower than that in H1975 cell. Meanwhile, the expression of Mcl-1 prominently
diminished in H3255 cell and the expression of Mcl-1 in H1975 cell had no obvious change in the presence of first generation
EGFR-TKIs Gefitinib. The expression of Mcl-1 was obviously decreased inH1975 cell after being treated with second generation
EGFR-TKIs Afatinib and third generation EGFR-TKIs AZD9291. Flow cytometry showed that specific siRNA which down-regulated the
expression of Mcl-1 in H1975 cell could promote the apoptosis.Conclusion:Mcl-1 participated in regulating the sensitivity to
EGFR-TKIs of NSCLC, and might be regarded as a target to overcome the resistance to EGFR-TKIs of NSCLC. |
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