| 秦彦昌 曾文 江伟 徐君 李维新△.可控性释放NGF的京尼平- 壳聚糖微球的研究[J].,2016,16(15):2824-2827 |
| 可控性释放NGF的京尼平- 壳聚糖微球的研究 |
| Study on Genipin-chitosan Microspheres for the Controlled Release of NerveGrowth Factor |
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| DOI： |
| 中文关键词: 壳聚糖 京尼平 微球 神经退行性疾病 |
| 英文关键词: Chitosan Genipin Microspheres Neurodegenerative disorders |
| 基金项目:国家自然科学基金项目（81402047） |
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| 中文摘要: |
| 目的：在体外研究京尼平- 壳聚糖微球可控性释放具有生物活性的神经生长因子的可行性。方法：采用" 乳化- 化学交联"
技术制备包埋神经生长因子的京尼平-壳聚糖微球，京尼平为化学交联剂；应用扫描电镜、粒径分布、体外缓释动力学及细胞生物
活性分别对微球的性能进行研究。结果：京尼平- 壳聚糖微球表面光滑，平均粒径在5.1～50.5 滋m 之间；京尼平的浓度可影响微
球在体外释放神经生长因子的速度，经高浓度京尼平交联的微球能减缓并持续释放神经生长因子；此外，从京尼平- 壳聚糖微球
释放的神经生长因子可维持PC12 细胞的生物活性，提高NGF生物利用率。结论：京尼平-壳聚糖微球能有效缓释具有生物活性
的NGF超过14 天，为神经退行性疾病的治疗提供一种治疗策略。 |
| 英文摘要: |
| Objective:To investigate the feasibility of the controlled release of bioactive nerve growth factor (NGF) with
genipin-chitosan microspheres in vitro.Methods:The genipin-chitosan microspheres were prepared by the emulsion-chemical cross-linking
method with genipin as a chemical cross-linking agent. Scanning electron microscopy, the size and distribution, release tests in vitro
and bioactivity assay were subsequently evaluated to study the functions of the microspheres.Results:The genipin-chitosan microspheres
had smooth surfaces with mean sizes between 5.1 and 50.5 um. The in vitro release profiles of NGF from genipin-chitosan microspheres
were influenced by the concentration of genipin. Genipin-chitosan microspheres were cross-linked with higher concentration of genipin,
which showed slower and sustained release of NGF. In addition, the released NGF from genipin-chitosan microspheres was capable of
maintaining the viability of PC12 cells and improving the biological utilization of NGF.Conclusion:Our findings suggest that NGF-loaded
genipin-chitosan microspheres are capable of releasing bioactive NGF over 14 days, and the result provide potential treatment strategy
in neurodegenerative disorders. |
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