| 陈林 王丹宁 伍峰 梁鑫 贺治青 梁春.洛伐他汀联用格列本脲可改善高糖诱导损伤的HUVEC 功能[J].,2016,16(12):2291-2295 |
| 洛伐他汀联用格列本脲可改善高糖诱导损伤的HUVEC 功能 |
| The Protective Effect of Lovastatin and Glyburide Combination on HighGlucose-induced Damage of HUVEC |
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| DOI： |
| 中文关键词: 糖尿病血管病变 洛伐他汀 格列本脲 人脐静脉血管内皮细胞 氧化应激 |
| 英文关键词: Diabetic Angiopathy Lovastatin Glyburide Human umbilical vein endothelial cells Oxidative stress |
| 基金项目:国家自然科学基金重点项目（81130065）；国家自然科学基金面上项目(31171130；81270405) |
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| 中文摘要: |
| 目的：探讨洛伐他汀与格列本脲联合用药对高糖诱导损伤的人脐静脉血管内皮细胞(HUVEC)的功能的影响及其机制。方法：
以体外培养至第三代的HUVEC 为研究对象，分四为：①空白对照组；②高糖组(33 mmol/L)；③高糖组(33 mmol/L)+ 洛伐他汀(2
umol/L)组；④高糖组(33 mmol/L)+洛伐他汀(2 umol/L)+格列本脲(0.2 umol/L)组。用transwell 小室法检测HUVEC的迁移功能，
用细胞流式仪检测HUVEC 内氧自由基(O2-)以及一氧化氮(NO)的表达水平。结果：与对照组相比，高糖组HUVEC 迁移功能明显
受损（P<0.01)；洛伐他汀单独用药组及其与格列本脲的联合用药组均能改善高糖损伤的HUVEC的迁移功能(均为P<0.01)。流式
结果显示，与对照组相比，高糖组HUVEC 内O2-水平明显增加而NO 表达水平明显降低(均为P<0.01)；两组给药组HUVEC 内
O2-水平及NO 表达水平相对于高糖组均有明显改善(均为P<0.01)。结论：洛伐他汀单独用药及其与格列本脲的联合用药均能改
善高糖损伤的HUVEC的迁移功能，可能与改善细胞内氧化应激水平有关。 |
| 英文摘要: |
| Objective:To investigate the effect of lovastatin and glyburide combination therapy on high glucose-induced damage
of human umbilical vein endothelial cells (HUVEC) function and its possible mechanism.Methods:The third generation of HUVEC
cultured in vitro were selected for the study, and divided into four groups as follows: ① control group; ② high glucose (33 mmol/L)
group; ③high glucose (33 mmol/L) + lovastatin (2 umol/L) group; ④high glucose (33 mmol/L) + lovastatin (2 umol/L) + glyburide (0.2
umol/L) group. The migration of HUVEC was observed with transwell chambers, and the level of oxygen free radicals (O2-) and nitric
oxide (NO) expression was detected by flow cytometer.Results:Compared with the control group, the migration of HUVEC was
obviously damaged in the high glucose group (P<0.01); compared with the high glucose group, the migration of HUVEC was obviously
improved in the groups of lovastain monotherapy and combination therapy with glibenclamide (P<0.01). Flow cytometry results showed
that the level of O2- expression in the HUVEC was increased and NO expression in the HUVEC was decreased in the high glucose group
compared with the control group (P<0.01); and the level of O2-and NO expression in the HUVEC was obviously improved in the groups
of lovastain monotherapy and combination therapy with glibenclamide (P<0.01).Conclusion:Lovastatin monotherapy and combination
therapy with glibenclamide's can improve the migration of HUVEC, which may be associated with improved intracellular oxidative stress
and NO levels. |
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