| 周霞 崔丽娜 杨琼 时永全 韩英.miR-1290对人脐带间充质干细胞增殖、周期和凋亡的调控作用研究[J].,2016,16(12):2213-2217 |
| miR-1290对人脐带间充质干细胞增殖、周期和凋亡的调控作用研究 |
| Effects of miR-1290 on Proliferation, Cell Cycle and Apoptosis of MSCs |
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| DOI： |
| 中文关键词: miR-1290 脐带间充质干细胞 增殖 细胞周期 凋亡 |
| 英文关键词: MiR-1290 MSCs Proliferation Cell cycle Apoptosis |
| 基金项目:国家自然科学基金青年基金项目（81400646）；国家重大新药创制项目（2014ZX09508002-001，2014ZX09104002-003） |
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| 中文摘要: |
| 探索microRNA-1290 对人脐带间充质干细胞增殖、细胞周期和凋亡的影响。方法：以从胎儿脐带分离的间充质干细胞
为基础细胞，构建miR-1290 模拟物，进行瞬时转染，通过荧光定量PCR 检测转染后miR-1290 水平；运用MTT 方法观察转染
miR-1290 后间充质干细胞的增殖情况；通过流式细胞仪染色观察转染后细胞周期和细胞凋亡的变化。结果：荧光定量PCR结果
显示，转染后miR-1290 表达水平显著升高12.2 倍；MTT 结果表明，高表达miR-1290 相比于阴性对照组（NC组）可以促进间充质
干细胞的增殖；流式细胞周期检测结果发现，转染miR-1290后，细胞周期G1期从(85.90± 1.91)%缩短至（76.35± 2.03)%，而S 期
和G2 期与对照组相比均明显增加，增殖指数(22.75± 3.01)相比于对照组（14.10± 1.87）也显著升高（P<0.05）；凋亡检测结果显示，
高表达miR-1290 细胞凋亡率（4.9± 0.276）%与阴性对照组（8.2± 0.891）%相比下降，有统计学意义（P<0.05）。结论：miR-1290可以
提高MSC 的增殖能力，延长S期和G2 期，并一定程度上抑制MSC 的凋亡。 |
| 英文摘要: |
| Objective:To explore the effect of miR-1290 on cell proliferation, cell cycle and apoptosis on human umbilical
ling-derived mesenchymal stem cell (MSCs).Methods:miR-1290 mimics was used to transfected into MSCs derived from human
umbilical cord bling. The expression level of miR-1290 after transfection was tested by fluorimetric determination PCR. The proliferation
ability was detected by MTT assay. Flow cytometry was applied to assess the cell cycle and apoptosis after transfection into MSCs.Results:RT-PCR results showed that the expression of miR-1290 was upregulated 12.2 fold after transfection. The MTT assay
demonstrated that overexpression of miR-1290 could promote the proliferation of MSCs significantly. Compared with control and
negative group(NC group), the period of G1 was decreased from(85.90± 1.91)%to (76.35± 2.03)%, while S and G2 period of cell cycle
were much longer. The proliferation index increased to (23.75± 3.01) compared wiith the control group (14.10± 1.87). The result of
apoptosis analyzed by flow cytometry showed that the rate of apoptosis of miR-1290（4.9± 0.276）%was reduced compared with the
negative control（8.2± 0.891）%, P<0.05.Conclusion:miR-1290 could enhance the proliferation ability of MSCs, decrease the period of
G1 and extend the S and G2 period, and repress the apoptosis of MSCs. |
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