| 严江波 黄林英 饶卓群 韩玉灿 邢怡桥 杨燕宁△.MEK/ERK信号通路对人结膜上皮细胞增殖的影响及其机制研究[J].,2016,16(11):2006-2009 |
| MEK/ERK信号通路对人结膜上皮细胞增殖的影响及其机制研究 |
| Effects and Mechanisms of MEK/ERK Signaling Pathway on theProliferation of HConEpiC |
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| DOI： |
| 中文关键词: MEK/ERK 信号通路 PD98059 人结膜上皮细胞 增殖 |
| 英文关键词: MEK/ERK signaling pathway PD98059 HConEpiC Proliferation |
| 基金项目:国家自然科学基金面上项目(81370986)；卫生部行业专项基金项目(201302015) |
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| 中文摘要: |
| 目的：探讨MEK/ERK信号通路对人结膜上皮细胞增殖的影响及其可能的机制。方法：采用不同浓度(0、12.5、25、50、100
umol/L)的MEK 抑制剂PD98059处理人结膜上皮细胞(HConEpiC)，通过CCK-8 法检测不同浓度PD98059 作用不同时间(0、12、
24、48 h)对人结膜上皮细胞增殖的影响，Western blot 检测不同浓度PD98059对人结膜上皮细胞ERK1/2、P-ERK1/2 表达的影响。
结果：相比对照组(0 umol/L)，不同浓度(12.5、25、50、100 umol/L)PD98059 处理后的人结膜上皮细胞增殖率明显下降，呈剂量- 效
应关系，且随处理时间增加(12、24、48 h)其抑制作用也显著增强，差异均有统计学意义(P<0.05)。不同浓度PD98059 处理人结膜上
皮细胞24 h后，其ERK 及p-ERK1/2 表达随处理浓度增加而降低，与对照组(0 滋mol/L)相比差异有统计学意义(P<0.05)，且二者表
达量与细胞增值抑制率均呈显著负相关（r=-0.995、r=-0.968, P<0.05）。结论：PD98059 可抑制人结膜上皮细胞增殖，这可能与其下
调ERK 表达和减少其活化有关。 |
| 英文摘要: |
| Objective:To explore the effects and possible mechanisms of MEK/ERK signaling pathway on the proliferation of human
conjunctival epithelial cells.Methods:Human conjunctival epithelial cells (HconEpiC) were treated with different concentrations (0,
12.5, 25, 50, 100 umol/L) of MEK inhibitor PD98059. CCK-8 method was used to detect the effect of different concentrations of
PD98059 on the proliferation of HconEpiC treated by different time periods (0, 12, 24, 48 h). Western-blot was used to measure the effect
of different concentrations of PD98059 on the expressions of ERK1/2, p-ERK1/2 protein.Results:Compared with the control group, the
proliferation of HConEpiC was significantly decreased by different concentrations (12.5, 25, 50, 100umol/L) of PD98059 in a concentrations
and time periods(0, 12, 24, 48 h) dependent manner (P<0.05). The ERK1/2 and p-ERK1/2 expressions of HConEpiC treated with
different concentrations of PD98059 were reduced (P<0.05), and the expressions of ERK and p-ERK were negatively correlated with the
inhibiting rates of HconEpiC proliferation (r=-0.995, r=-0.968, P<0.05).Conclusion:PD98059 could inhibit the proliferation of
HConEpiC, which might be associated with the inhibition of ERK expresson and activation. |
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